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You are on: EVA Portal / Viral cDNAs and partial sequences / Expression clone - Dengue 2 methyltransferase
 

  Expression clone - Dengue 2 methyltransferase

Item Category: Viral cDNAs and partial sequences
Expression clone of the Nter Domain of ns5 encoding the the methyltransferase with a 6his tag encoding sequence at its 5' -shorter and corrected construct compared to PMID
In stock

NOTIFICATION: This item is related to a virus which is classified in the BSL 2 category.

Region encompassed in this Product:
1-807 of ns5

  • Availability: In stock
  • Unit Definition: plasmid prep aliquot
  • Sequence:
  • Observed Mutations: none
  • Tag: His Nterm
  • Plasmid: pDEST
  • Plasmid Selection: Ampicillin
  • Encompassed Region: 1-807 of ns5
  • Related scientific publication >> PMID: 12032088




    Complementary Information about the virus related to the current Item:

  • Virus Family: Flaviviridae
  • Virus Genus: Flavivirus
  • Virus Name: Dengue virus 2
  • Strain: New Guinea
  • Subspecies: 2
  • Biosafety Level: 2
  • Host Type: Virus infects during its life cycle arthropod and vertebrate hosts. Virus infects Homo sapiens (human).
  • Morphology: Virions consist of an envelope and a nucleocapsid. Virus capsid is enveloped. Virions are spherical and measure 40-60 nm in diameter. Surface projections are small spikes surrounded by a prominent fringe. Capsid/nucleocapsid is round and exhibits polyhedral symmetry. The core is isometric and has a diameter of 25-30 nm.
  • Segmented Genome: NO
  • Nucleic Acid: Thegenome is not segmented and contains a single molecule of linear positive-sense, single-stranded RNA. The complete genome is 11000 nucleotides long. Sequence can be accessed from EBI-EMBL, or GenBank. The RNA is fully sequenced, complete sequence is 10703 nucleotides long. NCBI reference genome has the accession number [NC_001474]. The 5'-end of the genome has a methylated nucleotide cap. The 3'-terminus has no poly (A) tract.


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