The aim of the European Virus Archive project is to create and mobilise a European network of high calibre centres with the appropriate expertise, to collect, amplify, characterise, standardise, authenticate, distribute and track, mammalian and other exotic viruses. The EVA project is establishing a web-based catalogue to advertise and distribute viruses in the collection as well as associated products. In addition the EVA network also produce associated reagents on demand, to laboratories throughout Europe and also worldwide.

Site Map | contact |
X

EVA: European Virus Archive

EVA: European Virus Archive

EVA: European Virus Archive

 

EVA Portal

My Cart

  • There are still no products in your Cart.
You are on: EVA Portal / Mice / TgHKL25
 

  TgHKL25

Item Category: Mice
Knock-in for the immunoglobulin heavy chain gene of the KL25 hybridoma
In stock

NOTIFICATION: This item is related to a virus which is classified in the BSL 2 category.

This item corresponds to a Genetically modified Model.
For details see supplementary materials to publication PMID 14569006 or http://www.pnas.org/content/100/22/12883.long

  • Availability: In stock
  • Unit Definition: As a standard shipment, two breeding pairs are provided. Availability depends on breeding performance and can vary.
  • Strain And Genetic Background: C57BL/6
  • Genetic Modification or Targeting: Targeted insertion of the VDJ elements of the LCMV-WE neutralizing antibody KL25 (see related product) into the heavy chain locus. A detailed description of the targeting strategy is available through the PNAS website (http://www.pnas.org/content/100/22/12883.long)
  • Model: Genetically modified
  • Hygiene Status: Specific pathogen-free
  • Homozygosity: homozygous for the targeted heavy chain locus
  • Description of the Genetic Modification: For details see supplementary materials to publication PMID 14569006 or http://www.pnas.org/content/100/22/12883.long
  • Related scientific publication >> PMID: 14569006




    Complementary Information about the virus related to the current Item:

  • Virus Family: Arenaviridae
  • Virus Genus: Arenavirus
  • Virus Name: Lymphocytic choriomeningitis virus
  • Strain: Armstrong Clone 13
  • Biosafety Level: 2
  • Host Type: Virus infects during its life cycle a variety of vertebrate hosts. Virus has an enzootic cycle and is transmitted from rodents to humans. Virus infects Homo sapiens (human, SuborderSciurognathi; Family Muridae; Subfamily Murinae; virus infects Genus Mus m
  • Isolation Host: Mus musculus
  • Tropism: Pantropic
  • Morphology: Virions consist of an envelope and a nucleocapsid. Virus capsid is enveloped. Virions are spherical to pleomorphic measuring (50-)110-130(-300) nm in diameter. The envelope surrounds probably two nucleocapsids; has surface projections. Surface projections are distinctive club-shaped peplomers that are spaced widely apart and covering evenly the surface. Surface projections are composed of one type of protein. Surface projections are 10 nm long. Host ribosomes are seen inside the envelope (in varying numbers). Nucleocapsid is elongated and exhibits helical symmetry. Virions consist of two nucleocapsids. The nucleocapsid is filamentous and forming a closed circle; has a "string of beads" appearance with a varying length with a length of 1000-1300 nm (L segment, 450-640 nm (S segment) and a width of 3-4 nm. Nucleocapsid contains a polymerase complex and a nucleoprotein complex. Nucleocapsids are isolated nucleocapsids, free of contaminating host ribosomes organized in closed circles and display a linear array of nucleosomal subunits. Diagram and electron micrograph of arenavirus.
  • Segmented Genome: YES
  • Nucleic Acid: The Mrof the genome constitutes 2% of the virion by weight. The genome is segmented and consists of two segments of linear, negative-strand single-stranded RNA with an ambisense coding strategy. The genome is transcriptional inactive. Minor species of non-genomic nucleic acid are also found in virions. The encapsidated nucleic acid is mainly of genomic origin, but virions may also contain subgenomic RNA and nucleic acid of host origin including three host rRNA and three subgenomic mRNA that are derived from genomic S RNA (for the precursor of protein N and the precursor of protein GPC), or L RNA (for the Z protein). RNA segments are not homologous. The complete genome is about 10000-11000 nucleotides long. Sequence can be accessed from EBI-EMBL and GenBank. The RNA-L is fully sequenced, complete sequence is 7229 nucleotides long and encodes the RNA-dependent RNA polymerase L gene and the matrix protein Z gene respectively. Sequence has the accession number [DQ361066]. RNA-S is fully sequenced, complete sequence is 3377 nucleotides long and encodes envelope glycoprotein (GP-C) and nucleoprotein (NP) genes has the accession number [DQ361065]. Nucleotide sequences at the 3'-terminus are largely complementary to similar regions on the 5' end. The 5'-end of the genome does not have cap. The 3'-terminus has conserved nucleotide sequences; in all segments and species of same genus; sequence has 19-30 nucleotides in length; in S RNA. The intergenic region has S a hairpin configuration (potential). The multipartite genome is encapsidated, each segment in a separate nucleocapsid, and the nucleocapsids are surrounded by one envelope. Each virion contains often segments of the genome in non-equimolar proportions (due to frequent packaging of S RNA strands).


The Following Items are also available

Home page | Site Map | Contact us | Legal mentions

Copyright: http://www.european-virus-archive.com  http://www.european-virus-archive.org  http://www.european-virus-archive.eu