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St. Louis encephalitis
NOTIFICATION: This virus is classified in the BSL 3 category. This St. Louis encephalitis virus is stored under Viral Storage Medium -80C conditions. It was tested for mycoplasmic content and do not contains any mycoplasm and was Partly sequenced.
- Availability: In stock
- Unit Definition: 1 Vial containing 1 ML
- Sequencing: Partly sequenced
- Passage: 2 passages since accession into NCPV.
- Storage Conditions: Viral Storage Medium -80C
- Mycoplasmic Content: No
- Production Cell Line: Vero Cells
- Technical Recommendation: MEM 2% fcs
- Identification Technique: PCR, Sequence.
Complementary Information about the virus related to the current Item:
- Virus Family: Flaviviridae
- Virus Genus: Flavivirus
- Virus Name: St. Louis encephalitis virus
- Lineage: Viruses»ssRNA viruses»ssRNA positive-strand viruses, no DNA stage»Flaviviridae»Flavivirus»Japanese encephalitis virus group
- Biosafety Level: 3
- Related information on ICTVDB >> ref: 00.026.0.01.044.
- Taxonomic Information on NCBI Taxonomy >> Taxonomic ID: 11080
- Host Type: Virus infects during its life cycle arthropod and vertebrate hosts.Virus has an enzootic cycle and is transmitted from its arthropod vector to birds and its arthropod vector to an incidental incompetent host (dead end host). Viral hosts belong to the Doma
- Isolation Host: human.Virus was isolated by Muckenfuss, R.S., Armstrong, C.,
- Morphology: Virions have a complex construction and consist of an envelope and a nucleocapsid. Virions are enveloped by a detergent sensitive lipoprotein before leaving the host cell. Virions are spherical and 50 nm in diameter.
The envelope surrounds one nucleocapsid and has surface projections. The surface projection proteins are antigenic which exhibit hemagglutinin activity. Surface projections form ring-like subunits and are 7 nm in diameter. Host ribosomes are not seen inside the envelope. A regular capsid structure is present. Capsid/nucleocapsid is round and exhibits icosahedral symmetry. The nucleocapsid is isometric. The capsid shells of virions are composed of a single layer. The capsid surface structure does not reveal a regular pattern with distinctive features and is maintained using conventional electron microscopy. The capsomer arrangement is not distinct. Nucleocapsid contains a nucleoprotein complex. Virion populations are comprised of particles of uniform size. Capsids all have the same appearance and only one species is recovered in preparations.
- Segmented Genome: NO
- Nucleic Acid: The Mrof the genome constitutes 6% of the virion by weight. The genome is monomeric; not segmented and consists of a single molecule of linear positive-sense single-stranded RNA. The genome is infectious. Minor species of genomic nucleic acid are not found; non-genomic nucleic acid are not found in virions. The complete genome is about 11000 nucleotides long. Sequence can be accessed at EBI-EMBL, GenBank, and DNA Data Bank of Japan; the RNA has been partially sequenced and complete sequence is 11000 nucleotides long and has the accession number [M16614] (strain MSI-7 (listed at NCBI as MSI.7 and EBI as MS1-7), translated from cDNA of the clones pA3 and p to viral RNA). The sequenced region is 4673 nucleotides long and encodes a polyprotein in a single continuous open reading frame. The mRNAs of the complete cds encode capsid, membrane, envelope and nonstructural proteins (NS1, NS2a, NS2b, NS3). The 5'-end of the genome has a cap. The 5'-terminus has no poly (C) tract. Each virion contains a single copy of the genome.
Reference(s) to sequence databases at GenBank and specialty databases: nucleotide sequences; complete genomes. Medline ID: [87122172].
- Isolation Date: 1992-11-09
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