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You are on: EVA Portal / Viruses / Polio 1 Mahoney
 

  Polio 1 Mahoney

Item Category: Viruses
Cell culture supernatant containing Polio 1 Mahoney
In stock

NOTIFICATION: This virus is classified in the BSL 2 category.

This Poliovirus[Mahoney] is stored under Viral Storage Medium -80C conditions.
It was not tested for mycoplasmic content and was Fully sequenced.

  • Availability: In stock
  • Unit Definition: not determined
  • Sequence:
  • Sequencing: Fully sequenced
  • Passage: Passaged once on Vero E6 cells
  • Infectivity: Virus can be diluted 1:1500, CPE after 24 hours
  • Storage Conditions: Viral Storage Medium -80C
  • Mycoplasmic Content: Not tested
  • Production Cell Line: Vero E6
  • Identification Technique: PCR and sequencing of VP2
  • Isolation Conditions: Dilute 1:1500 incubate for 24 hours at 37°C using DMEM medium supplied with 10% FCS




    Complementary Information about the virus related to the current Item:

  • Virus Family: Picornaviridae
  • Virus Genus: Enterovirus
  • Virus Name: Poliovirus
  • Strain: Mahoney
  • Lineage: Viruses»ssRNA viruses»ssRNA positive-strand viruses, no DNA stage»Picornavirales»Picornaviridae»Enterovirus
  • Biosafety Level: 2
  • Related information on ICTVDB >> ref: 00.052.0.01.001.
  • Taxonomic Information on NCBI Taxonomy >> Taxonomic ID: 138953
  • Host Type: Virus infects during its life cycle a single type of vertebrate host. Virus infects Homo sapiens (human).
  • Isolation Host: Human
  • Morphology: Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 28-30 nm. Capsids appear round. The capsid consists of 12 capsomers. Capsids can be penetrated by stain and some appear dark in the center. Incomplete particles are common. They are devoid of nucleic acid characterized by capsids with dark centers in negative stain preparations. 3D image of virus reconstruction can be found at Institute for Molecular Virology, Madison, WI. Mahoney type 1 native empty capsid, Mahoney type 1 Poliovirus-2 Lansing, complexed with antiviral angent SCH48973 Poliovirus 3, Sabin strain Capsid structures, detailed structural and computational analysis are found in the Protein Data Bank (PDB) using VIPERdB, the VIrus Particle ExploreR Mahoney type 1; Mahoney type 1; native empty capsid, Mahoney type 1; Poliovirus-2 Lansing Complexed with antiviral angent SCH48973; Poliovirus 3, Sabin strain
  • Segmented Genome: YES
  • Nucleic Acid: The Mrof the genome constitutes 29.2% of the virion by weight. The genome is not segmented and contains a single molecule of linear positive-sense, single-stranded RNA. The complete genome is 7400 nucleotides long, is fully sequenced. Sequence has the accession number [V01150]. The 5'-end of the genome has a genome-linked protein (VPg). The 3'-terminus has a poly (A) tract.
  • Related Genome Reference >> Refseq:V01149.1


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