The aim of the European Virus Archive project is to create and mobilise a European network of high calibre centres with the appropriate expertise, to collect, amplify, characterise, standardise, authenticate, distribute and track, mammalian and other exotic viruses. The EVA project is establishing a web-based catalogue to advertise and distribute viruses in the collection as well as associated products. In addition the EVA network also produce associated reagents on demand, to laboratories throughout Europe and also worldwide.
NOTIFICATION: This virus is classified in the BSL 3 category.
This SARS coronavirus Frankfurt 1[Frankfurt-Frankfurt 1.] is stored under Viral Storage Medium -80C conditions. It was tested for mycoplasmic content and do not contains any mycoplasm and was Fully sequenced.
Availability: In stock
Unit Definition: not determined
Sequence:
Sequencing: Fully sequenced
Passage: Three times end point passaged
Viral Titre: 1.0e+7 not determined
Infectivity: Infection with MOI of 0,001 leads to a peak in viral load within 48 to 72 hours
Storage Conditions: Viral Storage Medium -80C
Mycoplasmic Content: No
Production Cell Line: Vero FM/Vero E6
Technical Recommendation: Handle under BSL3 conditions wearing a respirator or FFP3 masks
Identification Technique: PCR and sequencing; Plaques assay
Isolation Technique: End point passaged
Isolation Conditions: DMEM 10% FCS incubate at 37°C for 24 to 48 hours
Complementary Information about the virus related to the current Item:
Isolation Host: human.Virus was isolated by Prof Dr Hans Wilhelm Doerr; Insi
Morphology: Virions consist of an envelope, spikes, and a
nucleocapsid.
Segmented Genome: NO
Nucleic Acid: Thegenome is not segmented and consists of a single molecule of
linear, positive-sense, single-stranded RNA. The complete genome is this length excludes the 3' poly-A tail 29727 nucleotides
long. The RNA has been fully sequenced and
complete sequence is 29727 nucleotides long; sequence can be accessed at
GenBank with the accession number
[AY291315]. The
3'-terminus has a poly (A) tract 13 nucleotides in length.
Reference to nucleotide sequence PubMed ID:
[12917450].
Biological Properties