Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.
Unit: 2 micrograms of each plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
Carnation Italian ringspot virus full-length cDNA cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNA
Unit: 2 micrograms plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
Cymbidium ringspot virus DI-3 full-length cDNA cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of biologically active RNA
Tomato bushy stunt virus satellite RNA L (satRNA L) full-length cDNA cloned in plasmid pUC18 under the control of a T7 promoter, allowing for transcription of biologically active RNA
Infectious clone: double-stranded DNA copy of the genome of Coxsackievirus A17 strain 67591 downstream a T7 promoter in a plasmid backbone. In vitro transcribed RNA produces the virus upon transfection of permissive cells. The plasmid can be amplified in E. coli.