Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions.
The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection
The service aims at offering the possibility to evaluate the outcome of an infection by a SARS-CoV-2 strain or variant in a susceptible cell line : evaluation of the susceptibility of a cell line, evaluation of the infectivity of a variant compared to a reference variant, evaluation of the effect of infection on a cellular pathway etc... The project can be refined upon request
Panel of Freeze-dried SARS-CoV-2 variants (Europe, UK, South Africa, Brazil, Delta).
Please note Brazilian variant can not be supplied to private entities
General presentation of lyophilization.
Access to BSL3 and BSL3+ laboratories: Reminder of biosafety and biosecurity procedures.
Overall presentation of our lab process in the framework of EVAg :
- Lyophilization process on virus strains.
Overall presentation of Cryotec lyophilizer for derived product preparation such as inactivated quality controls:
- Lyophilization process on derived products.
Infectivity test from viral culture to qRT-PCR.