EVAg

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

Production on request of stabilisated RNA positif control, as known as Armored RNA for RT-PCR. This service includes: - Design of the positif control sequence based on the sequence provided by the costumer - Production and lyophilisation of Armored RNA according the quantity needed - LOD/quantification - Shipment of the Armored RNA at room temperature

Virus Neutralization Tests (VNT), also including plaque-reduction and foci-inhibition assays like PRNT and RFFIT, are the gold standard for determining antibody titers against viruses, and are essential serological tools. In this 5-day training the application and basic principles of VNTs will be discussed, and will be combined with wet lab training. The wet lab training will include all steps of the VNT, including analysis and interpretation of the results.

Generation of recombinant chimeric monoclonal antibodies: murine monoclonal antibodies (hybridoma cells or total RNA have to be provided by the end-user) will be sequenced, cloned, recombinantly expressed in Expi293 cells, and purified via protein G. Available backbones for heavy and light chain constant regions are: mouse IgG1, Ig kappa; mouse IgG2a, Ig kappa; rabbit IgG, Ig kappa; human IgG1, Ig kappa; porcine IgG, Ig kappa; and bovine IgG, Ig kappa. Other backbones could possibly also generated uppon request.

RG2 virus production (available on the EVAg catalogue) in single batch upon request containing 30 freeze-dried vials. The specific detection system (5 vials) by real-time PCR will be provided in the same package.