Plasmid contains the entire genome of HIV-1 with a specific mutation in the protease gene, which has been described as a polymorphic mutation. It is unclear, whether it can confer drug resistance to specific HIV-1 PR inhibitors directly. It has been shown in cell culture that the presence of the 63P change leads to higher "replicative fitness" of the virus. The respective mutations have been reported in viruses from HIV-infected individuals on PR inhibitors with virologic therapy failure.
The 3' terminal 217 nts of Aeonium ringspot virus RNA1 and RNA2 (excluding the poly(A) tail) are cloned in pGEM-T Easy allowing for transcription of a riboprobe to detect both genomic RNAs.
Unit: 2 micrograms of plasmid DNA, allowing for the synthesis of a probe after T7-driven transcription
The full-length genome of CpCDV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants
Unit: 1 microgram, allowing infection of plants following transformation into Agrobacterium
The full-length genome of TYLCSV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants
Unit: 1 microgram, allowing infection of plants following transformation into Agrobacterium
Gp41 recombinant, carrying the "MIF" motif, typical for subtype AE, in the fusion domain of the subtype B prototype NL4-3 - for studying the fusion function.
Plasmid contains the entire genome of HIV-1 with specific mutations in the reverse transcriptase gene, which can confer drug resistance to specific HIV-1 inhibitors. The respective mutations have been reported in viruses from HIV-infected individuals on reverse transcriptase inhibitors with virologic therapy failure.
Plasmid contains the entire genome of HIV-1 with specific mutations in the reverse transcriptase gene, which can confer drug resistance to specific HIV-1 inhibitors. The respective mutations have been reported in viruses from HIV-infected individuals on reverse transcriptase inhibitors with virologic therapy failure.
Plasmid contains the entire genome of HIV-1 with specific mutations in the reverse transcriptase gene, which can confer drug resistance to specific HIV-1 inhibitors. The respective mutations have been reported in viruses from HIV-infected individuals on reverse transcriptase inhibitors with virologic therapy failure.
proviral HIV-1 clone with an inserted TAATGA double stop codon in the endodomain of gp41 after position 8368 (NL4-3); (a stop codon at analogous position have been reported in early reports of SIV propagation in vitro.)
Deletion mutant of HIV-1 with a deleted env gene; RRE reintroduced for the expression of HIV-1 Gag. The genes vpr, env, tat, rev, vpu, nef are not expressed.
