Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions.
The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection
Freeze-dried Chikungunya virus, strain UVE/CHIKV/2009/FR/15.3 WT. This strain was obtained from modified infectious clone (LR2006_OPY1; La Reunion island 2006), transfected and cultivated on Vero cells
Freeze-dried Yellow fever virus, strain UVE/YFV/1927/GN/Asibi, complete ORF sequenced . It was produced by ISA method from "AY640589" genbank sequence.
Freeze-dried Zika virus, strain Dak84, african strain, complete genome sequenced . It was produced by ISA method from "A.taylori-tc/SEN/1984/41662-DAK" genbank sequence (KU955592)
Freeze-dried Zika virus, strain MAS66, asian strain, complete genome sequenced . It was produced by ISA method from "ZIKV/Aedes aegypti/MYS/P6-740/1966" genbank sequence (KX694533.2)
