EVAg

Human Immunodeficiency Virus (HIV) drug resistance remains a significant health issue that challenges treatment and prevention. The objective of this 3-week course is to learn bioinformatics techniques for the analysis of next-generation HIV sequencing results. This covers both HIV-1 and HIV-2. Teaching objectives include data curation, alignment, phylogenetics for typing, identification of drug resistance mutations, production of a resistance score, and interpretation. This course does not include genotyping itself, only sequence analysis and interpretation, as described above.

Plasmid containing an NL4-3 based HIV-1 genome with a big deletion between SalI site (5785) and XhoI site (8887), leading to a deletion of the viral genes vpu,env, tat, rev. Also the "rev-response-element" RRE is missing, leading to a lack of expression also of the gag-pol genes