EVAg

RNA extracted from infected cell culture supernatant

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

HCV 5`ncr Plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens.

Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.

The 3' terminal 217 nts of Aeonium ringspot virus RNA1 and RNA2 (excluding the poly(A) tail) are cloned in pGEM-T Easy allowing for transcription of a riboprobe to detect both genomic RNAs.

Viral RNA extracted from infected cell culture lysate.

Human parainfluenzavirus 1 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Herpes simplex virus Plasmid for real time PCR. The target region of a specific real time assay was amplified and cloned.

Human parainfluenza virus 4 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Plasmid for JC virus real time PCR. The target region of a specific real time assay was amplified and cloned.

Human parainfluenza virus 3

Target region of a specific real time assay was amplified and cloned.

Human Adenovirus plasmid for real time PCR. The target region of a specific real time assay was amplified and cloned.

Human parainfluenza virus 2 plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

Target region of a specific real time assay was amplified and cloned.

in vitro transcribed RNA for a specific Sapovirus real time PCR.

Plasmid for a specific human parvovirus B19 real time PCR. The target region of a specific real time assay was amplified and cloned.

Human respiratory syncytial virus plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

in vitro transcribed RNA for a specific human parainfluenza virus 2 real time PCR.

Invitro transcript for Hepatitis C virus genomic 5`ncr region assays. The target region of a specific real time assay was amplified and cloned.

Internal control plasmid for in vitro transcription for Human Immunodeficiency virus assays.

In vitro transcribed RNA for a specific HIV-1 real time PCR.

HIV-1 5'LTR Plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.

HCV X-tail Plasmid for in vitro transcription. The target region of a specific real time assay was amplified and cloned.