EVAg

This four day course will go through the general principles for the diagnosis of bovine leukemia virus, through a combination of discussions, demonstrations and hands-on training. The training will include sample processing, PCR, ELISA (serology), reagent production, virus related bio-safety and diagnostic quality systems.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

This service is intended for a global analysis of viral reference samples in order to obtain information about the genomic sequences on the one hand, and on the other hand, it opens the possibility to check the purity at the same time in order to exclude possible contaminations. It is used for quality control and certification of reference material.

Access to RIVM infrastructure to use anonymized human fecal material containing fully sequenced norovirus strains, representing different genotypes. Strains/genotypes/year (2012-2023) selection on demand. Custom-made package includes individual fecal material from 5 different patients, 10-20% suspended in virus culture medium. Service includes technical input on protocols and experiment design and support in the laboratory. Individual questions to refine potential requests can be sent to RIVM-EVAglobal@rivm.nl.

Access to RIVM infrastructure to use anonymized human fecal material containing fully sequenced sapovirus strains, representing different genotypes. Strains/genotypes/year (2012-2023) selection on demand. Custom-made package includes individual fecal material from 2 different patients, 10-20% suspended in virus culture medium. Service includes technical input on protocols and experiment design and support in the laboratory. Individual questions to refine potential requests can be sent to RIVM-EVAglobal@rivm.nl.

Virus Neutralization Tests (VNT), also including plaque-reduction and foci-inhibition assays like PRNT and RFFIT, are the gold standard for determining antibody titers against viruses, and are essential serological tools. In this 5-day training the application and basic principles of VNTs will be discussed, and will be combined with wet lab training. The wet lab training will include all steps of the VNT, including analysis and interpretation of the results.

The detection of viral pathogens infecting a given plant sample, obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology or for the certification of seed and planting material. The service offers the individual analysis of three plant samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

Development and production of Diagnostic systems with positive control for arboviruses (RUO)

Production on request of stabilisated RNA positif control, as known as Armored RNA for RT-PCR. This service includes: - Design of the positif control sequence based on the sequence provided by the costumer - Production and lyophilisation of Armored RNA according the quantity needed - LOD/quantification - Shipment of the Armored RNA at room temperature

The service aims at offering the possibility to evaluate the outcome of an infection by a SARS-CoV-2 strain or variant in a susceptible cell line : evaluation of the susceptibility of a cell line, evaluation of the infectivity of a variant compared to a reference variant, evaluation of the effect of infection on a cellular pathway etc... The project can be refined upon request

Specific tests for certain regulated viruses are required for the certification of seed and planting material. However, the main limitation of this approach is that only these viruses are detected. Latent and/or unknown viruses, which can also have a significant impact on yield and product quality, remain unidentified. Especially in vegetative propagation material, this inevitably leads to the accumulation of such pathogens. High-throughput sequencing and bioinformatics analysis offers a global analysis of the phytosanitary status of plants.

Generation of recombinant chimeric monoclonal antibodies: murine monoclonal antibodies (hybridoma cells or total RNA have to be provided by the end-user) will be sequenced, cloned, recombinantly expressed in Expi293 cells, and purified via protein G. Available backbones for heavy and light chain constant regions are: mouse IgG1, Ig kappa; mouse IgG2a, Ig kappa; rabbit IgG, Ig kappa; human IgG1, Ig kappa; porcine IgG, Ig kappa; and bovine IgG, Ig kappa. Other backbones could possibly also generated uppon request.

In this two-week course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus typing and molecular characterization will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: sample preparation, nucleic acid extraction, rolling-circle amplification, RFLP analysis, cloning, sequencing, phylogenetic analysis.

In this five-day course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus diagnosis will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: tissue-printing hybridization, dot-blot hybridization, preparation of non-radioactive probes, rapid sample extraction. After this course participants should have sufficient knowledge to introduce routine diagnostics of geminiviruses in their own laboratory.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting a plant or fungal sample, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed

In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens.

High-throughput sequencing and bioinformatics analysis of samples for a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens. Can be used for the discovery of novel or rare virus variants, the characterization of the genetic variation and the population composition.

Access to AMU/AFMB infrastructure for learning/training on techniques related to viral recombinant proteins

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

SARS-CoV-2 diagnostic training, including differential diagnostics of human Coronaviruses and other respiratory pathogens, incl. BSL-2 and BLS-3 conditions.

RG2 virus production (available on the EVAg catalogue) in single batch upon request containing 30 freeze-dried vials. The specific detection system (5 vials) by real-time PCR will be provided in the same package.

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and subject to availability practical application of the biosafety and biosecurity management at Institut Pasteur. Among others, establishment and validation of suitable decontamination methods for waist, rooms and material, as well as documentation and information management will be addressed.

In this 3-day course the basic principles of the protein microarray will be addressed by a combination of lectures, demonstration and hands-on training. The protein microarray technique can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.). The training encompasses wet lab training but also the analysis and interpretation of assay results. Special attention will be given to specific issues of Influenza and flavivirus cross reactivity.