recombinant N-terminal domain (NTD, aa 17-304) of SARS-CoV-2 SpyTag/StrepTag, glycosylated


EVAg

Produced by
: FLI
Shipping From
: Greifswald - DE

Product Description

Ref-SKU:
022P-04124
Recombinant expressed and purified N-terminal Domain (NTD) of SARS-CoV-2 for serological assays. The Protein was expressed in Expi293 cells and purified via Strep-Tactin XT and provided in Elution Buffer ( 100mM Tris-HCl; pH 8.0, 150mM NaCl, 1mM EDTA, 50mM Biotin). Additionally, the Protein contains a SpyTag for generation of multimeric scaffolds using a Spycatcher on Lumazine-Synthase-particles.
Product Risk Group: 

Information on the related virus

Virus name: 


Unit definition: 
700 µg in 500 µl

In stock

400,00 €
(Cost per access for Academics)

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Domain: 
Single domain
Genbank reference: 
Expression system: 
Mamalian cells
TAG: 
Expressed as: 
Soluble
TAG status of the solubilized protein: 
Uncleaved Tag
Protein purification: 
>95%
Functional characterization: 
Functionally characterized
Type of functional Characterization: 
Antigenic
Functional/Technical description: 
Derived from Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1
Shipping conditions: 
IATA Classification: 
Virus host type: 

Information about the origin of the product

Biological material origin: 
Synthetic origin
Modifications from the reference sequence(s): 
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s): 
C-terminal: Addition of a 25aa linker sequence (25 aa, GGSQSDSRGGNGNGGGAGGNGGGSA), SpyTag (15 aa, AHIVMVDAYKPTK), linker (6 aa, GGSGGS), and 1x StrepTag (8 aa, WSHPQFEK)