LOCUS       INP066       12819 bp DNA     circular SYN 21-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 21, 2024 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     source          1..12819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) of HIV-1"
     misc_feature    682..806
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.2"
                     /note="/other=misc_feature"
     CDS             790..2292
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.4"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQDPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2085..5096
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     SDRQGTVSFSFPQITLWQRPLVTIKIGGQLREALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGFDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     CDS             2253..2549
                     /codon_start=1
                     /label=protease
                     /label=pol protease
                     /note="/created_by=public1"
                     /translation="PQITLWQRPLVTIKIGGQLREALLDTGADDTVLEEMNLPGRWKPK
                     MIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF"
     gene            2549..4029
                     /label=RT
                     /note="/Color=#ff5555"
                     /note="/created_by=public1"
     gene            4030..4389
                     /label=RNAseH
                     /note="/Color=#ffddff"
                     /note="/created_by=public1"
     gene            4390..5093
                     /label=INT
                     /note="/Color=#bbbbff"
                     /note="/created_by=public1"
     CDS             5039..5615
                     /label=vif
     CDS             5558..5845
                     /label=vpr
     CDS             5829..6044
                     /label=tat exon 1
     CDS             6060..6302
                     /label=vpu
     CDS             6221..7489
                     /codon_start=1
                     /label=env
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=79.1"
                     /note="/match_length=100.5"
                     /note="/other=CDS"
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPVW
                     KEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVEQM
                     HEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFNIST
                     SIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFA
                     ILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANFTDNAKT
                     IIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNATL
                     KQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWFNSTWS
                     TEGSNNTEGSDTITLPCRIKQFIN"
     gene            7099..7207
                     /label=V3
                     /note="/Color=#3333ff"
                     /note="/created_by=public1"
     CDS             7749..8783
                     /codon_start=1
                     /product="gp41 Envelope gene"
                     /label=gp41
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="AVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLL
                     RAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWS
                     NKSLEQIWNNMTWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFN
                     ITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGI
                     EEEGGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEA
                     LKYWWNLLQYWSQELKNSAVNLLNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGL
                     ERILL"
     misc_feature    7760..7993
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8788..9105
                     /label=nef
     LTR             9077..9710
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10586..11174)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(join(11345..12136,12137..12205))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12206..12310)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(12647..12713)
                     /codon_start=1
                     /label=lacZ-alpha
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=CDS"
                     /translation="LA**RRGPHRSPFPTVAQPEWR"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatttgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggat
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
     2281 tcgtcacaat aaagataggg gggcaactaa gggaagctct attagataca ggagcagatg
     2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa
     2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata
     2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
     2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
     2581 aattaaagcc cgggatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
     2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
     2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
     2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
     2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
     2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
     2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
     3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
     3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
     3121 ttgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
     3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
     3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
     3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
     3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
     3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
     3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
     3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
     3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
     3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
     3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
     3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
     3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
     3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
     3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
     4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
     4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
     4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
     4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
     4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
     4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
     4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
     4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
     4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
     4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
     4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
     4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
     4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
     4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
     5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
     5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
     5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
     5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
     5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
     5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
     5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
     5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
     5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
     5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
     5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
     5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
     5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
     5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
     5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
     5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
     6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
     6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
     6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta
     6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat
     6301 ttgtagtgct acagaaaaat tgtgggtcac agtctattat ggggtacctg tgtggaagga
     6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa
     6421 tgtttgggcc acacatgcct gtgtacccac agaccccaac ccacaagaag tagtattggt
     6481 aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga tgcatgagga
     6541 tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt
     6601 tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta gcgggagaat
     6661 gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa gcataagaga
     6721 taaggtgcag aaagaatatg cattctttta taaacttgat atagtaccaa tagataatac
     6781 cagctatagg ttgataagtt gtaacacctc agtcattaca caggcctgtc caaaggtatc
     6841 ctttgagcca attcccatac attattgtgc cccggctggt tttgcgattc taaaatgtaa
     6901 taataagacg ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca
     6961 tggaatcagg ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga
     7021 tgtagtaatt agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa
     7081 cacatctgta gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat
     7141 ccagagggga ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc
     7201 acattgtaac attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt
     7261 aagagaacaa tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc
     7321 agaaattgta acgcacagtt ttaattgtgg aggggaattt ttctactgta attcaacaca
     7381 actgtttaat agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga
     7441 aggaagtgac acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga
     7501 agtaggaaaa gcaatgtatg cccctcccat cagtggacaa attagatgtt catcaaatat
     7561 tactgggctg ctattaacaa gagatggtgg taataacaac aatgggtccg agatctttca
     7621 gacctggagg aggcgatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7681 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7741 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7801 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctgatatag
     7861 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7921 cagtctgggg catcaaacag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7981 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8041 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaataac atgacctgga
     8101 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8161 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8221 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8281 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8341 ggcagggata ttcaccatta tcgtttcaga cccacctccc aatcccgagg ggacccgaca
     8401 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     8461 tgaacggatc attagcactt atctgggacg atctgcggag cctgtgcctc ttcagctacc
     8521 accgcttgag agacttactc ttgattgtaa cgaggattgt ggaacttctg ggacgcaggg
     8581 ggtgggaagc cctcaaatat tggtggaatc tcctacagta ttggagtcag gaactaaaga
     8641 atagtgctgt taacttgctc aatgccacag ccatagcagt agctgagggg acagataggg
     8701 ttatagaagt attacaagca gcttatagag ctattcgcca catacctaga agaataagac
     8761 agggcttgga aaggattttg ctataagatg ggtggcaagt ggtcaaaaag tagtgtgatt
     8821 ggatggcctg ctgtaaggga aagaatgaga cgagctgagc cagcagcaga tggggtggga
     8881 gcagtatctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctaac
     8941 aatgctgctt gtgcctggct agaagcacaa gaggaggaag aggtgggttt tccagtcaca
     9001 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9061 aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9121 ttgtggattt accacacaca aggctacttc cctgattggc agaactacac accagggcca
     9181 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     9241 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     9301 ggaatggatg accctgagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     9361 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgaca tcgagcttgc
     9421 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     9481 tggcgagccc tcagatgctg catataagca gctgcttttt gcctgtactg ggtctctctg
     9541 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9601 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9661 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca cccaggaggt
     9721 agaggttgca gtgagccaag atcgcgccac tgcattccag cctgggcaag aaaacaagac
     9781 tgtctaaaat aataataata agttaagggt attaaatata tttatacatg gaggtcataa
     9841 aaatatatat atttgggctg ggcgcagtgg ctcacacctg cgcccggccc tttgggaggc
     9901 cgaggcaggt ggatcacctg agtttgggag ttccagacca gcctgaccaa catggagaaa
     9961 ccccttctct gtgtattttt agtagatttt attttatgtg tattttattc acaggtattt
    10021 ctggaaaact gaaactgttt ttcctctact ctgataccac aagaatcatc agcacagagg
    10081 aagacttctg tgatcaaatg tggtgggaga gggaggtttt caccagcaca tgagcagtca
    10141 gttctgccgc agactcggcg ggtgtccttc ggttcagttc caacaccgcc tgcctggaga
    10201 gaggtcagac cacagggtga gggctcagtc cccaagacat aaacacccaa gacataaaca
    10261 cccaacaggt ccaccccgcc tgctgcccag gcagagccga ttcaccaaga cgggaattag
    10321 gatagagaaa gagtaagtca cacagagccc tgcattaatg aatcggccaa cgcgcgggga
    10381 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    10441 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    10501 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    10561 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    10621 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    10681 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    10741 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    10801 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    10861 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    10921 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    10981 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    11041 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    11101 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    11161 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    11221 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    11281 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    11341 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    11401 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    11461 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    11521 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11581 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11641 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11701 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11761 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    11821 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    11881 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    11941 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    12001 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    12061 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    12121 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    12181 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    12241 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    12301 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
    12361 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
    12421 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    12481 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    12541 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
    12601 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
    12661 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc caggcatcag
    12721 agtccttggt gtggagggag ggaccagcgc agcttccagc catccacctg atgaacagaa
    12781 cctagggaaa gccccagttc tacttacacc aggaaaggc
//
