LOCUS       INP068       12818 bp DNA     circular SYN 21-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 21, 2024 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     source          1..12818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) of HIV-1"
     misc_feature    682..806
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.2"
                     /note="/other=misc_feature"
     CDS             790..2292
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.4"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQDPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2085..5096
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     SDRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEVNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     gene            4030..4389
                     /label=RNAseH
                     /note="/Color=#ffddff"
                     /note="/created_by=public1"
     gene            4390..5093
                     /label=INT
                     /note="/Color=#bbbbff"
                     /note="/created_by=public1"
     CDS             5039..5615
                     /label=vif
     CDS             5558..5845
                     /label=vpr
     CDS             5829..6044
                     /label=tat exon 1
     CDS             6221..8782
                     /codon_start=1
                     /label=env
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=97.7"
                     /note="/match_length=99.8"
                     /note="/other=CDS"
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPVW
                     KEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVEQM
                     HEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFNIST
                     SIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFA
                     ILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANFTDNAKT
                     IIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNATL
                     KQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFYCNSTQLFNSTWFNSTWS
                     TEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGN
                     NNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNMTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVNGSLAL
                     IWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVNL
                     LNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGLERILL"
     CDS             6304..7743
                     /codon_start=1
                     /label=Env gp120
                     /note="gp120 Env coding sequence"
                     /translation="*CYRKIVGHSLLWGTCVEGSNHHSILCIRC*SI*YRGT*CLGHTC
                     LCTHRPQPTRSSIGKCDRKF*HVEK*HGRTDA*GYNQFMGSKPKAMCKINPTLC*FKVH
                     *FEE*Y*YQ***RENDNGERRDKKLLFQYQHKHKR*GAERICILL*T*YSTNR*YQL*V
                     DKL*HLSHYTGLSKGIL*ANSHTLLCPGWFCDSKM***DVQWNRTMYKCQHSTMYTWNQ
                     ASSINSTAVKWQSSRRRCSN*ICQFHRQC*NHNSTAEHICRN*LYKTQQQYKKKYPYPE
                     GTRESICYNRKNRKYETSTL*H**SKMECHFKTDS*QIKRTIWK**NNNL*AILRRGPR
                     NCNAQF*LWRGIFLL*FNTTV**YLV**YLEY*RVK*H*RK*HNHTPMQNKTIYKHVAG
                     SRKSNVCPSHQWTN*MFIKYYWAAINKRWW**QQWVRDLQTWRRRYEGQLEK*II*I*S
                     SKN*TIRSSTHQGKEKSGAERK"
     gene            7099..7207
                     /label=V3
                     /note="/Color=#3333ff"
                     /note="/created_by=public1"
     CDS             7748..8782
                     /codon_start=1
                     /product="gp41 Envelope gene"
                     /label=gp41
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="AVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLL
                     RAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWS
                     NKSLEQIWNNMTWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFN
                     ITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGI
                     EEEGGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEA
                     LKYWWNLLQYWSQELKNSAVNLLNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGL
                     ERILL"
     misc_feature    7759..7992
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8787..9104
                     /label=nef
     LTR             9076..9709
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     rep_origin      10585..11173
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(join(11344..12135,12136..12204))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        12205..12309
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             complement(12646..12712)
                     /codon_start=1
                     /label=lacZ-alpha
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=CDS"
                     /translation="LA**RRGPHRSPFPTVAQPEWR"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggat
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
     2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagataca ggagcagatg
     2341 atacagtatt agaagaagtg aatttgccag gaagatggaa accaaaaatg atagggggaa
     2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata
     2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
     2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
     2581 aattaaagcc cgggatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
     2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
     2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
     2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
     2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
     2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
     2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
     3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
     3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
     3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
     3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
     3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
     3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
     3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
     3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
     3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
     3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
     3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
     3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
     3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
     3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
     3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
     3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
     3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
     4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
     4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
     4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
     4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
     4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
     4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
     4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
     4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
     4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
     4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
     4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
     4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
     4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
     4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
     5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
     5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
     5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
     5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
     5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
     5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
     5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
     5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
     5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
     5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
     5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
     5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
     5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
     5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
     5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
     5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
     6061 atgcaaccta taatagtagc aatagtagca ttagtagtag caataataat agcaatagtt
     6121 gtgtggtcca tagtaatcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
     6181 ttaattgata gactaataga aagagcagaa gacagtggca atgagagtga aggagaagta
     6241 tcagcacttg tggagatggg ggtggaaatg gggcaccatg ctccttggga tattgatgat
     6301 ctgtagtgct acagaaaaat tgtgggtcac agtctattat ggggtacctg tgtggaagga
     6361 agcaaccacc actctatttt gtgcatcaga tgctaaagca tatgatacag aggtacataa
     6421 tgtttgggcc acacatgcct gtgtacccac agaccccaac ccacaagaag tagtattggt
     6481 aaatgtgaca gaaaatttta acatgtggaa aaatgacatg gtagaacaga tgcatgagga
     6541 tataatcagt ttatgggatc aaagcctaaa gccatgtgta aaattaaccc cactctgtgt
     6601 tagtttaaag tgcactgatt tgaagaatga tactaatacc aatagtagta gcgggagaat
     6661 gataatggag aaaggagaga taaaaaactg ctctttcaat atcagcacaa gcataagaga
     6721 taaggtgcag aaagaatatg cattctttta taaacttgat atagtaccaa tagataatac
     6781 cagctatagg ttgataagtt gtaacacctc agtcattaca caggcctgtc caaaggtatc
     6841 ctttgagcca attcccatac attattgtgc cccggctggt tttgcgattc taaaatgtaa
     6901 taataagacg ttcaatggaa caggaccatg tacaaatgtc agcacagtac aatgtacaca
     6961 tggaatcagg ccagtagtat caactcaact gctgttaaat ggcagtctag cagaagaaga
     7021 tgtagtaatt agatctgcca atttcacaga caatgctaaa accataatag tacagctgaa
     7081 cacatctgta gaaattaatt gtacaagacc caacaacaat acaagaaaaa gtatccgtat
     7141 ccagagggga ccagggagag catttgttac aataggaaaa ataggaaata tgagacaagc
     7201 acattgtaac attagtagag caaaatggaa tgccacttta aaacagatag ctagcaaatt
     7261 aagagaacaa tttggaaata ataaaacaat aatctttaag caatcctcag gaggggaccc
     7321 agaaattgta acgcacagtt ttaattgtgg aggggaattt ttctactgta attcaacaca
     7381 actgtttaat agtacttggt ttaatagtac ttggagtact gaagggtcaa ataacactga
     7441 aggaagtgac acaatcacac tcccatgcag aataaaacaa tttataaaca tgtggcagga
     7501 agtaggaaaa gcaatgtatg cccctcccat cagtggacaa attagatgtt catcaaatat
     7561 tactgggctg ctattaacaa gagatggtgg taataacaac aatgggtccg agatcttcag
     7621 acctggagga ggcgatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7681 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7741 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7801 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctgatatagt
     7861 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7921 agtctggggc atcaaacagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7981 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8041 ttggaatgct agttggagta ataaatctct ggaacagatt tggaataaca tgacctggat
     8101 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8161 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8221 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8281 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8341 gcagggatat tcaccattat cgtttcagac ccacctccca atcccgaggg gacccgacag
     8401 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     8461 gaacggatca ttagcactta tctgggacga tctgcggagc ctgtgcctct tcagctacca
     8521 ccgcttgaga gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg
     8581 gtgggaagcc ctcaaatatt ggtggaatct cctacagtat tggagtcagg aactaaagaa
     8641 tagtgctgtt aacttgctca atgccacagc catagcagta gctgagggga cagatagggt
     8701 tatagaagta ttacaagcag cttatagagc tattcgccac atacctagaa gaataagaca
     8761 gggcttggaa aggattttgc tataagatgg gtggcaagtg gtcaaaaagt agtgtgattg
     8821 gatggcctgc tgtaagggaa agaatgagac gagctgagcc agcagcagat ggggtgggag
     8881 cagtatctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctaaca
     8941 atgctgcttg tgcctggcta gaagcacaag aggaggaaga ggtgggtttt ccagtcacac
     9001 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9061 aagaaaaggg gggactggaa gggctaattc actcccaaag aagacaagat atccttgatt
     9121 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     9181 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     9241 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     9301 gaatggatga ccctgagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     9361 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgacat cgagcttgct
     9421 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     9481 ggcgagccct cagatgctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     9541 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9601 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9661 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccaggaggta
     9721 gaggttgcag tgagccaaga tcgcgccact gcattccagc ctgggcaaga aaacaagact
     9781 gtctaaaata ataataataa gttaagggta ttaaatatat ttatacatgg aggtcataaa
     9841 aatatatata tttgggctgg gcgcagtggc tcacacctgc gcccggccct ttgggaggcc
     9901 gaggcaggtg gatcacctga gtttgggagt tccagaccag cctgaccaac atggagaaac
     9961 cccttctctg tgtattttta gtagatttta ttttatgtgt attttattca caggtatttc
    10021 tggaaaactg aaactgtttt tcctctactc tgataccaca agaatcatca gcacagagga
    10081 agacttctgt gatcaaatgt ggtgggagag ggaggttttc accagcacat gagcagtcag
    10141 ttctgccgca gactcggcgg gtgtccttcg gttcagttcc aacaccgcct gcctggagag
    10201 aggtcagacc acagggtgag ggctcagtcc ccaagacata aacacccaag acataaacac
    10261 ccaacaggtc caccccgcct gctgcccagg cagagccgat tcaccaagac gggaattagg
    10321 atagagaaag agtaagtcac acagagccct gcattaatga atcggccaac gcgcggggag
    10381 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10441 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10501 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10561 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10621 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10681 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10741 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10801 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10861 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10921 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10981 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    11041 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11101 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11161 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11221 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    11281 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    11341 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    11401 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    11461 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    11521 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    11581 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11641 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11701 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11761 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11821 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11881 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11941 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    12001 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    12061 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    12121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    12181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    12241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    12301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    12361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    12421 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    12481 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    12541 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    12601 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    12661 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc aggcatcaga
    12721 gtccttggtg tggagggagg gaccagcgca gcttccagcc atccacctga tgaacagaac
    12781 ctagggaaag ccccagttct acttacacca ggaaaggc
//
