LOCUS       INP079       12817 bp DNA     circular SYN 22-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 22, 2024 from SnapGene 7.1.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     source          1..12817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) of HIV-1"
                     /note="color: #ccffff"
     misc_feature    682..806
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.2"
                     /note="/other=misc_feature"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             790..2291
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.3"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIGLPTREGQGIFFRADQSQQPH
                     QKRASGLGKRQQLPLRSRIR*TRNCIL*LPSDHSLAATHRHN"
     CDS             2085..5095
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="FFREDWPSHKGRPGNFLQSRPEPTAPPEESFRFGEETTTPSQKQD
                     PIDKELYPLASLRSLFGSDPSSQ*R*GGN*RKL**IQEQMIQY*KK*ICQEDGNQKS*G
                     ELEVLSK*DSMIRYP*KSADIKL*VQY**DLHLST*LEEIC*LRLAAL*IFPLVLLRLY
                     Q*N*SPGWMAQKLNNGH*QKKK*KH**KFVQKWKRKEKFQKLGLKIHTILQYLP*RKKT
                     VLNGEN**ISENLIRELKISGKFN*EYHILQG*NRKNQ*QYWMWAMHIFQFP*IKTSGS
                     ILHLPYLV*TMRHQGLDISTMCFHRDGKDHQQYSSVA*QKS*SLLENKIQT*SSINTWM
                     ICM*DLT*K*GSIEQK*RN*DNIC*GGDLPHQTKNIRKNLHSFGWVMNSILINGQYSL*
                     CCQKRTAGLSMTYRN*WEN*IGQVRFMQGLK*GNYVNFLGEPKH*QK*YH*QKKQS*NW
                     QKTGRF*KNRYMECIMTHQKT**QKYRSRGKANGHIKFIKSHLKI*KQESMQE*RVPTL
                     MM*NN*QRQYKK*PQKA**YGERLLNLNYPYKRKHGKHGGQSIGKPPGFLSGSLSIPLP
                     **SYGTS*RKNP**EQKLSM*MGQPIGKLN*EKQDM*LTEEDKKLSP*RTQQIRRLSYK
                     QFI*LCRIRD*K*T**QTHNMHWESFKHNQIRVNQS*SVK**SS**KRKKSTWHGYQHT
                     KELEEMNK*INWSVLESGKYYF*ME*IRPKKNMRNITVIGEQWLVILTYHL**QKK**P
                     AVINVS*KGKPCMDK*TVAQEYGS*IVHI*KEKLSW*QFM*PVDI*KQK*FQQRQGKKQ
                     HTSS*N*QEDGQ*KQYIQTMAAISPVLQLRPPVGGRGSSRNLAFPTIPKVKE**NL*IK
                     N*RKL*DR*EIRLNILRQQYKWQYSSTILKEKGGLGGTVQGKE**T**QQTYKLKNYKN
                     KLQKFKIFGFITGTAEIQFGKDQQSSSGKVKGQ**YKIIVT*K*CQEEKQRSSGIMENR
                     WQVMIVWQVDRMRI"
     CDS             2252..2548
                     /codon_start=1
                     /label=protease
                     /label=pol protease
                     /note="/created_by=public1"
                     /note="color: #ccffff"
                     /translation="PQITLWQRPIVTIKIGGQLKEALIDTGADDTVLEEVNLPGRWKPK
                     IIGGIGGFVKVRQYDQIPIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF"
     CDS             2548..4028
                     /codon_start=1
                     /label=RT
                     /note="/Color=#ff5555"
                     /note="/created_by=public1"
                     /note="color: #ccffff"
                     /translation="SH*SY*DCTSKIKARDGWPKS*TMAIDRRKNKSISRNLYRNGKGR
                     KNFKNWA*KSIQYSSICHKEKRQY*MEKISRFQRT**ENSRFLGSSIRNTTSCRVKTEK
                     ISNSTGCGRCIFFSSLR*RLQEVYCIYHT*YKQ*DTRD*ISVQCASTGMERITSNIPV*
                     HDKNLRAF*KTKSRHSHLSIHG*FVCRI*LRNRAA*NKNRGTETTSVEVGIYHTRQKTS
                     ERTSIPLDGL*TPS**MDSTAYSAARKGQLDCQ*HTEISGKIELGKSDLCRD*SKAIM*
                     TS*GNQSTNRSSTTNRRSRARTGRKQGDSKRTGTWSVL*PIKRLNSRNTEAGARPMDIS
                     NLSRAI*KSENRKVCKNEGCPH**CETINRGSTKNSHRKHSNMGKDS*I*ITHTKGNMG
                     SMVDRVLASHLDS*VGVCQYPSLSEVMVPVRERTHNRSRNFLCRWGSQ*GN*IRKSRIC
                     N*QRKTKSCPPNGHNKSED*VTSNSSSFAGFGIRS"
     gene            4029..4388
                     /label=RNAseH
                     /note="/Color=#ffddff"
                     /note="/created_by=public1"
                     /note="color: #ccffff; direction: RIGHT"
     gene            4389..5092
                     /label=INT
                     /note="/Color=#bbbbff"
                     /note="/created_by=public1"
                     /note="color: #ccffff; direction: RIGHT"
     CDS             5038..5614
                     /label=vif
                     /note="color: #ccffff; direction: RIGHT"
     CDS             5557..5844
                     /label=vpr
                     /note="color: #ccffff; direction: RIGHT"
     CDS             5828..6043
                     /label=tat exon 1
                     /note="color: #ccffff; direction: RIGHT"
     CDS             6059..6301
                     /label=vpu
                     /note="color: #ccffff; direction: RIGHT"
     CDS             6220..8781
                     /codon_start=1
                     /label=env
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=97.5"
                     /note="/match_length=99.8"
                     /note="/other=CDS"
                     /note="color: #993366"
                     /translation="MRVKEKYQHLWRWGWKWGTMLLGILMICSATEKLWVTVYYGVPVW
                     KEATTTLFCASDAKAYDTEVHNVWATHACVPTDPNPQEVVLVNVTENFNMWKNDMVEQM
                     HEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDTNTNSSSGRMIMEKGEIKNCSFNIST
                     SIRDKVQKEYAFFYKLDIVPIDNTSYRLISCNTSVITQACPKVSFEPIPIHYCAPAGFA
                     ILKCNNKTFNGTGPCTNVSTVQCTHGIRPVVSTQLLLNGSLAEEDVVIRSANFTDNAKT
                     IIVQLNTSVEINCTRPNNNTRKSIRIQRGPGRAFVTIGKIGNMRQAHCNISRAKWNATL
                     KQIASKLREQFGNNKTIIFKQSSGGDPEIVTHSFNCGGEFFHCNSTQLFNSTWFNSTWS
                     TEGSNNTEGSDTITLPCRIKQFINMWQEVGKAMYAPPISGQIRCSSNITGLLLTRDGGN
                     NNNGSEIFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNNMTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGIEEEGGERDRDRSIRLVNGSLAL
                     IWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEALKYWWNLLQYWSQELKNSAVNL
                     LNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGLERILL"
     CDS             6303..7742
                     /codon_start=1
                     /label=Env gp120
                     /note="gp120 Env coding sequence"
                     /note="color: #ccffff"
                     /translation="*CYRKIVGHSLLWGTCVEGSNHHSILCIRC*SI*YRGT*CLGHTC
                     LCTHRPQPTRSSIGKCDRKF*HVEK*HGRTDA*GYNQFMGSKPKAMCKINPTLC*FKVH
                     *FEE*Y*YQ***RENDNGERRDKKLLFQYQHKHKR*GAERICILL*T*YSTNR*YQL*V
                     DKL*HLSHYTGLSKGIL*ANSHTLLCPGWFCDSKM***DVQWNRTMYKCQHSTMYTWNQ
                     ASSINSTAVKWQSSRRRCSN*ICQFHRQC*NHNSTAEHICRN*LYKTQQQYKKKYPYPE
                     GTRESICYNRKNRKYETSTL*H**SKMECHFKTDS*QIKRTIWK**NNNL*AILRRGPR
                     NCNAQF*LWRGIFPL*FNTTV**YLV**YLEY*RVK*H*RK*HNHTPMQNKTIYKHVAG
                     SRKSNVCPSHQWTN*MFIKYYWAAINKRWW**QQWVRDLQTWRRRYEGQLEK*II*I*S
                     SKN*TIRSSTHQGKEKSGAERK"
     gene            7098..7206
                     /label=V3
                     /note="/Color=#3333ff"
                     /note="/created_by=public1"
                     /note="color: #ccffff; direction: RIGHT"
     CDS             7747..8781
                     /codon_start=1
                     /product="gp41 Envelope gene"
                     /label=gp41
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #ccffff"
                     /translation="AVGIGALFLGFLGAAGSTMGAASMTLTVQARQLLSDIVQQQNNLL
                     RAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWS
                     NKSLEQIWNNMTWMEWDREINNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFN
                     ITNWLWYIKLFIMIVGGLVGLRIVFAVLSIVNRVRQGYSPLSFQTHLPIPRGPDRPEGI
                     EEEGGERDRDRSIRLVNGSLALIWDDLRSLCLFSYHRLRDLLLIVTRIVELLGRRGWEA
                     LKYWWNLLQYWSQELKNSAVNLLNATAIAVAEGTDRVIEVLQAAYRAIRHIPRRIRQGL
                     ERILL"
     misc_feature    7758..7991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ccffff"
     CDS             8786..9103
                     /label=nef
                     /note="color: #ccffff; direction: RIGHT"
     LTR             9075..9708
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ccffff"
     rep_origin      10584..11172
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
                     /note="color: #ffff00"
     CDS             complement(join(11343..12134,12135..12203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11343 .. 12134 / #ccffff
                        2: 12135 .. 12203 / #ccffff / signal sequence
                     Cleavage site after base 12134"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        12204..12308
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
                     /note="color: #ffffff"
     CDS             complement(12645..12711)
                     /codon_start=1
                     /label=lacZ-alpha
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=38.5"
                     /note="/other=CDS"
                     /note="color: #ccffff"
                     /translation="LA**RRGPHRSPFPTVAQPEWR"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatt
     2101 ggccttccca caagggaagg ccagggaatt ttcttcagag cagaccagag ccaacagccc
     2161 caccagaaga gagcttcagg tttggggaag agacaacaac tccctctcag aagcaggatc
     2221 cgatagacaa ggaactgtat cctttagctt ccctcagatc actctttggc agcgacccat
     2281 cgtcacaata aagatagggg ggcaattaaa ggaagctcta atagatacag gagcagatga
     2341 tacagtatta gaagaagtga atttgccagg aagatggaaa ccaaaaatca tagggggaat
     2401 tggaggtttt gtcaaagtaa gacagtatga tcagataccc atagaaatct gcggacataa
     2461 agctataggt acagtattag taggacctac acctgtcaac ataattggaa gaaatctgtt
     2521 gactcagatt ggctgcactt taaattttcc cattagtcct attgagactg taccagtaaa
     2581 attaaagccc gggatggatg gcccaaaagt taaacaatgg ccattgacag aagaaaaaat
     2641 aaaagcatta gtagaaattt gtacagaaat ggaaaaggaa ggaaaaattt caaaaattgg
     2701 gcctgaaaat ccatacaata ctccagtatt tgccataaag aaaaaagaca gtactaaatg
     2761 gagaaaatta gtagatttca gagaacttaa taagagaact caagatttct gggaagttca
     2821 attaggaata ccacatcctg cagggttaaa acagaaaaaa tcagtaacag tactggatgt
     2881 gggcgatgca tatttttcag ttcccttaga taaagacttc aggaagtata ctgcatttac
     2941 catacctagt ataaacaatg agacaccagg gattagatat cagtacaatg tgcttccaca
     3001 gggatggaaa ggatcaccag caatattcca gtgtagcatg acaaaaatct tagagccttt
     3061 tagaaaacaa aatccagaca tagtcatcta tcaatacatg gatgatttgt atgtaggatc
     3121 tgacttagaa atagggcagc atagaacaaa aatagaggaa ctgagacaac atctgttgag
     3181 gtggggattt accacaccag acaaaaaaca tcagaaagaa cctccattcc tttggatggg
     3241 ttatgaactc catcctgata aatggacagt acagcctata gtgctgccag aaaaggacag
     3301 ctggactgtc aatgacatac agaaattagt gggaaaattg aattgggcaa gtcagattta
     3361 tgcagggatt aaagtaaggc aattatgtaa acttcttagg ggaaccaaag cactaacaga
     3421 agtagtacca ctaacagaag aagcagagct agaactggca gaaaacaggg agattctaaa
     3481 agaaccggta catggagtgt attatgaccc atcaaaagac ttaatagcag aaatacagaa
     3541 gcaggggcaa ggccaatgga catatcaaat ttatcaagag ccatttaaaa atctgaaaac
     3601 aggaaagtat gcaagaatga agggtgccca cactaatgat gtgaaacaat taacagaggc
     3661 agtacaaaaa atagccacag aaagcatagt aatatgggga aagactccta aatttaaatt
     3721 acccatacaa aaggaaacat gggaagcatg gtggacagag tattggcaag ccacctggat
     3781 tcctgagtgg gagtttgtca atacccctcc cttagtgaag ttatggtacc agttagagaa
     3841 agaacccata ataggagcag aaactttcta tgtagatggg gcagccaata gggaaactaa
     3901 attaggaaaa gcaggatatg taactgacag aggaagacaa aaagttgtcc ccctaacgga
     3961 cacaacaaat cagaagactg agttacaagc aattcatcta gctttgcagg attcgggatt
     4021 agaagtaaac atagtgacag actcacaata tgcattggga atcattcaag cacaaccaga
     4081 taagagtgaa tcagagttag tcagtcaaat aatagagcag ttaataaaaa aggaaaaagt
     4141 ctacctggca tgggtaccag cacacaaagg aattggagga aatgaacaag tagataaatt
     4201 ggtcagtgct ggaatcagga aagtactatt tttagatgga atagataagg cccaagaaga
     4261 acatgagaaa tatcacagta attggagagc aatggctagt gattttaacc taccacctgt
     4321 agtagcaaaa gaaatagtag ccagctgtga taaatgtcag ctaaaagggg aagccatgca
     4381 tggacaagta gactgtagcc caggaatatg gcagctagat tgtacacatt tagaaggaaa
     4441 agttatcttg gtagcagttc atgtagccag tggatatata gaagcagaag taattccagc
     4501 agagacaggg caagaaacag catacttcct cttaaaatta gcaggaagat ggccagtaaa
     4561 aacagtacat acagacaatg gcagcaattt caccagtact acagttaagg ccgcctgttg
     4621 gtgggcgggg atcaagcagg aatttggcat tccctacaat ccccaaagtc aaggagtaat
     4681 agaatctatg aataaagaat taaagaaaat tataggacag gtaagagatc aggctgaaca
     4741 tcttaagaca gcagtacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     4801 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4861 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     4921 agatccagtt tggaaaggac cagcaaagct cctctggaaa ggtgaagggg cagtagtaat
     4981 acaagataat agtgacataa aagtagtgcc aagaagaaaa gcaaagatca tcagggatta
     5041 tggaaaacag atggcaggtg atgattgtgt ggcaagtaga caggatgagg attaacacat
     5101 ggaaaagatt agtaaaacac catatgtata tttcaaggaa agctaaggac tggttttata
     5161 gacatcacta tgaaagtact aatccaaaaa taagttcaga agtacacatc ccactagggg
     5221 atgctaaatt agtaataaca acatattggg gtctgcatac aggagaaaga gactggcatt
     5281 tgggtcaggg agtctccata gaatggagga aaaagagata tagcacacaa gtagaccctg
     5341 acctagcaga ccaactaatt catctgcact attttgattg tttttcagaa tctgctataa
     5401 gaaataccat attaggacgt atagttagtc ctaggtgtga atatcaagca ggacataaca
     5461 aggtaggatc tctacagtac ttggcactag cagcattaat aaaaccaaaa cagataaagc
     5521 cacctttgcc tagtgttagg aaactgacag aggacagatg gaacaagccc cagaagacca
     5581 agggccacag agggagccat acaatgaatg gacactagag cttttagagg aacttaagag
     5641 tgaagctgtt agacattttc ctaggatatg gctccataac ttaggacaac atatctatga
     5701 aacttacggg gatacttggg caggagtgga agccataata agaattctgc aacaactgct
     5761 gtttatccat ttcagaattg ggtgtcgaca tagcagaata ggcgttactc gacagaggag
     5821 agcaagaaat ggagccagta gatcctagac tagagccctg gaagcatcca ggaagtcagc
     5881 ctaaaactgc ttgtaccaat tgctattgta aaaagtgttg ctttcattgc caagtttgtt
     5941 tcatgacaaa agccttaggc atctcctatg gcaggaagaa gcggagacag cgacgaagag
     6001 ctcatcagaa cagtcagact catcaagctt ctctatcaaa gcagtaagta gtacatgtaa
     6061 tgcaacctat aatagtagca atagtagcat tagtagtagc aataataata gcaatagttg
     6121 tgtggtccat agtaatcata gaatatagga aaatattaag acaaagaaaa atagacaggt
     6181 taattgatag actaatagaa agagcagaag acagtggcaa tgagagtgaa ggagaagtat
     6241 cagcacttgt ggagatgggg gtggaaatgg ggcaccatgc tccttgggat attgatgatt
     6301 tgtagtgcta cagaaaaatt gtgggtcaca gtctattatg gggtacctgt gtggaaggaa
     6361 gcaaccacca ctctattttg tgcatcagat gctaaagcat atgatacaga ggtacataat
     6421 gtttgggcca cacatgcctg tgtacccaca gaccccaacc cacaagaagt agtattggta
     6481 aatgtgacag aaaattttaa catgtggaaa aatgacatgg tagaacagat gcatgaggat
     6541 ataatcagtt tatgggatca aagcctaaag ccatgtgtaa aattaacccc actctgtgtt
     6601 agtttaaagt gcactgattt gaagaatgat actaatacca atagtagtag cgggagaatg
     6661 ataatggaga aaggagagat aaaaaactgc tctttcaata tcagcacaag cataagagat
     6721 aaggtgcaga aagaatatgc attcttttat aaacttgata tagtaccaat agataatacc
     6781 agctataggt tgataagttg taacacctca gtcattacac aggcctgtcc aaaggtatcc
     6841 tttgagccaa ttcccataca ttattgtgcc ccggctggtt ttgcgattct aaaatgtaat
     6901 aataagacgt tcaatggaac aggaccatgt acaaatgtca gcacagtaca atgtacacat
     6961 ggaatcaggc cagtagtatc aactcaactg ctgttaaatg gcagtctagc agaagaagat
     7021 gtagtaatta gatctgccaa tttcacagac aatgctaaaa ccataatagt acagctgaac
     7081 acatctgtag aaattaattg tacaagaccc aacaacaata caagaaaaag tatccgtatc
     7141 cagaggggac cagggagagc atttgttaca ataggaaaaa taggaaatat gagacaagca
     7201 cattgtaaca ttagtagagc aaaatggaat gccactttaa aacagatagc tagcaaatta
     7261 agagaacaat ttggaaataa taaaacaata atctttaagc aatcctcagg aggggaccca
     7321 gaaattgtaa cgcacagttt taattgtgga ggggaatttt tccactgtaa ttcaacacaa
     7381 ctgtttaata gtacttggtt taatagtact tggagtactg aagggtcaaa taacactgaa
     7441 ggaagtgaca caatcacact cccatgcaga ataaaacaat ttataaacat gtggcaggaa
     7501 gtaggaaaag caatgtatgc ccctcccatc agtggacaaa ttagatgttc atcaaatatt
     7561 actgggctgc tattaacaag agatggtggt aataacaaca atgggtccga gatcttcaga
     7621 cctggaggag gcgatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7681 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7741 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7801 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tgatatagtg
     7861 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7921 gtctggggca tcaaacagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7981 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     8041 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaataacat gacctggatg
     8101 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     8161 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     8221 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     8281 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     8341 cagggatatt caccattatc gtttcagacc cacctcccaa tcccgagggg acccgacagg
     8401 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     8461 aacggatcat tagcacttat ctgggacgat ctgcggagcc tgtgcctctt cagctaccac
     8521 cgcttgagag acttactctt gattgtaacg aggattgtgg aacttctggg acgcaggggg
     8581 tgggaagccc tcaaatattg gtggaatctc ctacagtatt ggagtcagga actaaagaat
     8641 agtgctgtta acttgctcaa tgccacagcc atagcagtag ctgaggggac agatagggtt
     8701 atagaagtat tacaagcagc ttatagagct attcgccaca tacctagaag aataagacag
     8761 ggcttggaaa ggattttgct ataagatggg tggcaagtgg tcaaaaagta gtgtgattgg
     8821 atggcctgct gtaagggaaa gaatgagacg agctgagcca gcagcagatg gggtgggagc
     8881 agtatctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaacaa
     8941 tgctgcttgt gcctggctag aagcacaaga ggaggaagag gtgggttttc cagtcacacc
     9001 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     9061 agaaaagggg ggactggaag ggctaattca ctcccaaaga agacaagata tccttgattt
     9121 gtggatttac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     9181 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     9241 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     9301 aatggatgac cctgagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     9361 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgacatc gagcttgcta
     9421 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     9481 gcgagccctc agatgctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     9541 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9601 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9661 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcacc caggaggtag
     9721 aggttgcagt gagccaagat cgcgccactg cattccagcc tgggcaagaa aacaagactg
     9781 tctaaaataa taataataag ttaagggtat taaatatatt tatacatgga ggtcataaaa
     9841 atatatatat ttgggctggg cgcagtggct cacacctgcg cccggccctt tgggaggccg
     9901 aggcaggtgg atcacctgag tttgggagtt ccagaccagc ctgaccaaca tggagaaacc
     9961 ccttctctgt gtatttttag tagattttat tttatgtgta ttttattcac aggtatttct
    10021 ggaaaactga aactgttttt cctctactct gataccacaa gaatcatcag cacagaggaa
    10081 gacttctgtg atcaaatgtg gtgggagagg gaggttttca ccagcacatg agcagtcagt
    10141 tctgccgcag actcggcggg tgtccttcgg ttcagttcca acaccgcctg cctggagaga
    10201 ggtcagacca cagggtgagg gctcagtccc caagacataa acacccaaga cataaacacc
    10261 caacaggtcc accccgcctg ctgcccaggc agagccgatt caccaagacg ggaattagga
    10321 tagagaaaga gtaagtcaca cagagccctg cattaatgaa tcggccaacg cgcggggaga
    10381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    11041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    11281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    11341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    11401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    11461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    11521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    11581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    11641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    11701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    11761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    11821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    11881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    11941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    12001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    12061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    12121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    12181 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    12241 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    12301 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    12361 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    12421 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    12481 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    12541 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    12601 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
    12661 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggcatcagag
    12721 tccttggtgt ggagggaggg accagcgcag cttccagcca tccacctgat gaacagaacc
    12781 tagggaaagc cccagttcta cttacaccag gaaaggc
//
