LOCUS       plasmid                10380 bp    DNA     circular SYN 29-MAY-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      complement(2634..2992)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(join(7266..10380,1..2288))
                     /note="pLannotate"
                     /label="L (fragment)"
                     /database="swissprot"
                     /identity="50.4"
                     /match_length="81.5"
                     /fragment="True"
                     /other="CDS"
     promoter        3019..3123
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3124..3984
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      4155..4743
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     terminator      complement(5251..5298)
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        5067..5097
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    5031..5052
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        5166..5184
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    5105..5121
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             2377..2532
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     CDS             5532..5840
                     /note="pLannotate"
                     /label="Z"
                     /database="swissprot"
                     /identity="57.7"
                     /match_length="104.0"
                     /fragment="False"
                     /other="CDS"
     ncRNA           complement(5360..5452)
                     /note="pLannotate"
                     /label="HDV ribozyme"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="102.2"
                     /fragment="False"
                     /other="ncRNA"
     CDS             complement(5306..5355)
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5957..7282)
                     /note="pLannotate"
                     /label="L (fragment)"
                     /database="swissprot"
                     /identity="55.4"
                     /match_length="19.9"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(2345..2362)
                     /note="pLannotate"
                     /label="T7 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.7"
                     /fragment="True"
                     /other="promoter"
     CDS             4926..5018
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    5197..5218
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="20.4"
                     /fragment="True"
                     /other="misc_feature"
ORIGIN
        1 agcatccttt catcttcatc cccctcttca gatgggttga tgttaatact gtcaaactcc
       61 aacttgggct ccaaatactt ctcaaagcac ttaatctgat ctgtcaacct atctggagtt
      121 tccttagtta taaaatgaca catgtatgat aggtttaaca caaacttaaa tctattagtc
      181 aacatagtgc tcaccccttc acccaggatg atgttgagaa tgctcctcac cacactatac
      241 aagaggtgct ccggctctgt tatgagatcc tccctcaact tttccattaa ctgcttatgg
      301 tggaagtctg aaacaaaggc cattatgaag tatcgcagac cttgcaagaa cttctgtgct
      361 cttttgctag ggttcacaag tatgaggatc atggtgattt tgagcaataa ttttaaagtt
      421 ttttgttgat ctctgagttc tatacattca gacaaccaac tcatcatggt gttgatagtc
      481 tcatagataa cacagtgtga gaaaattgca ggaaaaaacc tttttggatc tgcatagaat
      541 gaacaaatct cccccttctc aggattgtta atggcataac attttgagca ctctcctgtt
      601 ttctgataaa ttaagttgaa attcccgctt cccatgataa agctttgatt aaaagcttcc
      661 ttacaacgta cacgtttgta ccttgcagca ccatactcat tttgcctcac tttggctact
      721 gatgatgtct tcattgaatt caccaatgcc aaagagatgc tagatagtct tctcaggtct
      781 tctatggaat cagcttccaa ctcaataaga gggtagggga attgattccc ctcttctttc
      841 tcatagctca ttgttggctg aatccctctt ttctccatta acataagcct ctcgtaactg
      901 tcctcctcca gtatatcttc ctctttaaca taatctctaa agcacaaact ggcagaggat
      961 aatgcctctt tggcctttgt gaggagtttg tttatgatgt caaagaatat aagctcagat
     1021 ctctgacaca gagttcctac acctcccttc ttcttcattg cactcaccat tctcctttca
     1081 atccactttt taaggtcctt ttgtgtttct tgtagtgaca ccaatctgtc gttcacactt
     1141 aagaatgagg agccaagcca ttctgatcct aacagctcgc cgtgacattt aagatccttt
     1201 aagtgggcca aaaggatgat tgcgtcaaaa gtcagcatta gttttcttct ggtgttcaat
     1261 aactttagtg attttatttt attcaacagt gacttccatc caggcacaga gctttggaag
     1321 tgattctgca agcagctgtc tcgatcaact ccctcttgcc tataacggtc gtagtcacca
     1381 tagagcgctg ttaccatagg cgaggatttt acaaacctct cttttagagg atcaatctct
     1441 gagggtgtgt ccccctctct gtcaatataa aggttgttga aatctctcaa gagctgtcgc
     1501 ctatctgtcc tgacaaattg tctttctatc tcaccctctt taagcttatt cctaaaagca
     1561 tgaaattctg atacaatctc ttttttcaca tcaagaatgg acattttgtt attgatgcct
     1621 ctgtggcaga gctccaacac cctctcataa tgatcagatc gagaatctgt caaaacattg
     1681 aggctctcaa tgcccgatgt tctcactcct gcttttgtta aagattcaca aagccttgag
     1741 tactcagatt cttcaaacat ttttgatgac tgctgcgaga attcaagtaa gctaaatagg
     1801 atatgcctaa acctctcatt ggcccattct ggaaacaaat cagtgtagaa attggttctg
     1861 ccgtctatca agggcaacag aatgatgtca acagatgata agtcaggctt taaagactct
     1921 agcttaatgg tatcttcttt atacttttgc tcaaagttta ttggtgaagt cctgacaaag
     1981 cattccagta atattaatgt gttcccactg aatttgaatc catctggcac cacaaatggt
     2041 agaccagggc acagaacacc ttgtctctgc agaaggattt caacagaaag gtcttctgag
     2101 ttgtgctcac aaccattggc tttgcaactg tctatctcaa tacagagtga caacaactta
     2161 aggccctcaa ttaatagcat tctcggctct gtctgaacta ggaaggctag cttctgtttt
     2221 gaaagcctct catcctctaa gaggtacctg ctcacaagat ctttgctctc ggacagcaac
     2281 tcctccatca ccacacactt gttcctcaga agagagagtc acaatgccta ggatcctcgg
     2341 tgcgctatag tgagtcgtat taagcttatc gcgcgctcac tggccgtcgt tttacaacgt
     2401 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2461 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2521 ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2581 gttaaatcag ctcatttttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2641 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2701 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2761 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2821 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2881 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2941 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     3001 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3061 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3121 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3181 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3241 gcacgagtgg gttacatcga actggatctc aacagtggta agatccttga gagttttcgc
     3301 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3361 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3421 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3481 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3541 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3601 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3661 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3721 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3781 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3841 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3901 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3961 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4021 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4261 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4801 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4861 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4921 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4981 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     5041 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     5101 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     5161 cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccct
     5221 agtggatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc
     5281 cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag cttcctttcg
     5341 ggctttgtta gcagccggat ccccgccctc ccttagccat ccgagtggac gtgcgtcctc
     5401 cttcggatgc ccaggtcgga ccgcgaggag gtggagatgc catgccgacc cgcgcaccgg
     5461 ggatcctagg caattttgtg gatctttgct gatacttcat tctgcactca aagccgcatc
     5521 cggcaagagt catggggaag acgcagtcca agggacagcc cagcacaaat ctcctcaagg
     5581 agacagaacc aaggcatcca gtaataccag atgccagggg aactggccct gaattctgca
     5641 agagctgctg gttcgaaagg agggggttag tcaggtgcaa cgatcactac ctatgtttga
     5701 actgtcttac acttttacac acagtgtcag ataggtgccc catctgcaaa cacaaactac
     5761 ccttcagact agagctccag acccagccga cagcaccacc agaaatccca ccatcccaaa
     5821 atcccccacc ctacagcccc tgaggcagaa ctccgccccg aagcccctcc caaacacccc
     5881 tcccctcccc cccgggggga ccccccgccg gggctccccc cggggggaga tgcgcgagct
     5941 gttctcggag cacctactca atgtcgaggg gaggccccac ctcttcaaag aggggcctgc
     6001 acagcctccc tcttaatcta aacttcccct ctgaagactg gatcatcagt gaagcctgcg
     6061 atctactgta gcagagacta tagcctttaa aatctgccca gaagtcaatt ccggagaaca
     6121 ctctagtaag gaagttttcg tagccttcag gcatgcatcg tcccacaacc cctataatat
     6181 caagttgtag ccagtgtagt ccttggtcca ttctggatct cagcatcttg ctcaaagatc
     6241 tctccaagat ttcggaatga tcctccatta gctcaccaat aaaaacatta agatcctggt
     6301 catggatgtt ggggttgaag ggcaggagtt tcctggtccc ttcaataagg tatcctgagt
     6361 caacaactaa ggggatttca acatgctcag gccctatatg cgtgaaatca tgttcctcca
     6421 ggtccaggat ctcctcattc aataagacgg acaagggtat cacatcagga atgggggaac
     6481 tatccctcaa gtagtcacaa atattttcag tggacagcaa ccaaattgat gtggtttccc
     6541 ttttaaacca tgtagatgtc tgtgcaactc tccagcaatc tttaattgcg gatctttgaa
     6601 cactaccact gtaagttaca tcaagggaat gaggcttgaa taatccacct tccaacacca
     6661 cccctttgaa aaggaggtgg agactaaatt tctctttcaa tgattcaccc tgacttctca
     6721 cctgaaaatt tactgtgaca gaaatttgaa cgctcccaac actgaggtcg gcaaaagctt
     6781 caagcagctc acttaccgac tcaaagacgt gctgctcggt taatcctgat gctatgagct
     6841 gtcctaagtt tagaattgct ggtgcacatt caaaatccag ccaagcataa cctgagtaaa
     6901 gatcatgcct gtacatgggt ctttcaacac ccttataaat aaccttttct ataaaagaag
     6961 aaaaggggcc gaggttgtca agcccttccg actcaggaat tgaagatcta tgtggaaacc
     7021 aagtaaaaga acctaaagtc ctgcttgttg caacgaatgg tctgatgaag gactcaaagt
     7081 agatttgtct caggaaattt tgtaccacat cataagtggt caccaccctt tcatccgtga
     7141 ttgggagatg agacctctca tgtgctattg ctaaatctga agacagaacc acataatgct
     7201 cttctctctt ccacttgaca acatgagaaa ttaatgacat tgcctcacaa ttcacatcta
     7261 gaaccacaca aagattcaag aaacttaatc cttggtgtcc acctcatttt cgttgatgct
     7321 aggtcactca tgaagggcaa gaggtgtctc tcaaatagag atgggtaaag tcttctgaat
     7381 gagtgaataa tatggttaaa ccccaccttg tcttccaaga gttttgtggt agtgggcttc
     7441 attgggaaat agtcacatgg cttgaaggga attgaggatg ttttctcctt cactttgggt
     7501 tcacccaaca cccaggaacc cagctcaagt gatgtagata aggctatgca gaagtaatcc
     7561 caaaggacag gtctcaagaa acctaacgct tcctcagctg cctttgtgtg ctccttaccc
     7621 aagatccaaa gatcgacgcc cttaactttc aagctggaaa cagaatccaa tgctgacagt
     7681 cttgaaacta atgattttat ataggcggtg gacctatctg agaatcgaac acatttgcta
     7741 cctagtgtct tgcaaagacc tatgaaacct gatgctatcc cactctgaaa ggcacttttg
     7801 ttgacggctt cacagagcaa cttttgtgcc cctcttgtga aattagagga taacttgctt
     7861 tgcaatgttt tcaccaaggt aggcagtttt tcttccccga gttcaacagc acctgtgtaa
     7921 atgaccttct gtctcatgac catctttaaa gggtgatgag ttgtcaaatt aagccaacac
     7981 accgataaat cctcttctga gagggtgtca tcaccaacaa ggctggtaag tttcttgaag
     8041 gaatctgcgg ggtctcctga caagtagatg gctgtgaatt cttcatgaag ctctcctaat
     8101 ttcagcttat taaaaactgt ccttaggacc ttcctaactt tctgagtgct ttctggtagg
     8161 attgcctcaa tgttcctcat aatcctatag cccctattgc catcaaccca atctttaaca
     8221 tcagaatcca aaaacaacaa gaaaggatca atgggatatt gtgcatatct aagtagattc
     8281 agtgttctct cctgaatagc gttacatagt gtgacaggca caccattggc cactgactga
     8341 tcaatgatag tgtcaatagt ttcagctaat tgatgtggct ccttacactt aacgttgtgg
     8401 agggcggcag ccacgaattt cgttaacaat ggaacttcat caccccagac ataaaacctt
     8461 gatttgaact cagcaacaaa tgaaccaata acactctttg gactgatgaa tttattcaat
     8521 tgatcactta ggaaatagtg aaattctaga atggcttgga attcctcagg ctcatttgag
     8581 agtaaatctg aaagatcctt gccaaatagt gatatttgat catcactaga ggtatatgaa
     8641 tcaacttcat cttcaaatag acacttaatg caataattta tgaatctctc actcactaga
     8701 ccgtagaagt cagaagcatt gtgcaagatc ccttgcccca tgtcaattat tgagctgaga
     8761 tgtgatggta tcctgttttg tttaaagtat tcaaagaaga aagcttctgt tgacgtttgg
     8821 tggagtgatt ttttcaaacc taacctagac ttaagataag atctcatcat tgcattcaca
     8881 acattgaaag gtacctcaat taacttgtga atgtgccacg acagcaaagt ggacacgtaa
     8941 tccttacttt ttatgtcctc tatatcatct cctgtctccc atcgtaaatt ttgaagaaac
     9001 accaggaaaa gaaaagggga catcataggc ccccacttgc tgtgatcgag gctataagag
     9061 acctgaccta gtgaaacatt caatttcatg gctagaatgg cattgtggaa ttccttttca
     9121 ttattaagac agctccctct atattgctta gttagtgcct caaaataatc ctcaattagg
     9181 cgtgtgaaca tttttgtcct caggtcacct atataaagtt ccctgttccc tccaacttgt
     9241 tctttgtaag ataaggaaaa cttgagcctt ccagtatcag gacccaagga tgtgtatgac
     9301 tgtggggatt cttgactgta aaaacatagg ttctttagaa ctgcagttgt acaatttgtc
     9361 aatgacagtg ctttacctaa tgcctcagaa ttgctctctc tttcactgat cctaacatca
     9421 ttcatcagcc tttctctatc aatcttgaag tttaagcatt tgtttttgta atggctgtat
     9481 tttccagcca atttccctgc attcatctga agaagtagtg acttgaagca ctgaaaaaac
     9541 tgctggtcat tgaatgttct ggtggagaca gcttgggtga tgcatgttat tgggcaagag
     9601 tccaaggaat ccaaatagaa atactttgac cttaactcca aatccttaaa aaagcctttg
     9661 cagatcatct cataagcatc tccaggtagt agtgactcat caaaatcttc caccatgtgg
     9721 tgtgctatct ctgcctggat cagtctgtag taaacacagc ccacatcttc agtacccaaa
     9781 accttcctca agtctgtcaa ggacttctcc tctgacccat ttcccacagg gaacttcctg
     9841 tcatatctat acttctcaag gacgacatca acagatttct tcacatcctc tagacagtca
     9901 agtgcacaac tatccaagaa taactcatct aattcgtcag gagccccttg tgaattggga
     9961 tttgaggaac ctgacttctt ccccacaaca aggtcactaa tggccttttg caccttataa
    10021 tcataatcct ccttactcaa gaggtattta gttttcctct ggaatacctc tgatagctgg
    10081 caaacagaag cagccactag tttatcatga tcgtaattaa gtatcctctc tccgtcagta
    10141 tacttattca caaccacact tttgttgctt gcaaggtcaa gagctgttgc acacccagca
    10201 gtaactaatg gatctttaaa cccacttagg tcatctcctt tcttgaaaag agagccattg
    10261 ttaaaagaag acgtcatcat tgagaagaat tttcttgaca caccaggaac tttataagat
    10321 attctgttgt tactcatact ggtttctttg gataaaaatt tgtttgctga ttcaagcagc
//
