LOCUS       Exported                4000 bp DNA     circular SYN 13-SEP-2021
DEFINITION  [P thai dans pTIT] Homologous recombination reaction between 
            [pTIT.txt] as a target sequence  and [PTHAI.xdna] as an inserted 
            sequence. - 4024 bp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 13, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     148..164
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        172..190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    241..702
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    707..1600
                     /label=P gene
     terminator      1686..1733
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1756..1772)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1809..1827)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1848..1864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1872..1888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1896..1926)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1941..1962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2250..2838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3009..3869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3870..3974)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcgcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
       61 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      121 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgcgcgc gtaatacgac
      181 tcactatagg gcgaattaat tccggttatt ttccaccata ttgccgtctt ttggcaatgt
      241 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
      301 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
      361 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
      421 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
      481 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
      541 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
      601 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
      661 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aataccatga gcaagatctt
      721 tgtcaatccg agcgcaatca gagccggttt ggctgatctc gaaatggctg aggagactgt
      781 tgatctaatc aatagaaaca tagaagacaa tcaagcccat ctccagggag aacccataga
      841 agtggataat ctccccgaag atatgaggcg acttcagctg gatgacagaa aaccgtctgg
      901 cctcagtggg atagctactg cggtagaaag caagtatagg gaggactttc agatggatga
      961 aggagaggac cctagtctct tattccagtc atacctagac aatgttggag tccaaatagt
     1021 cagacagatg aggtcaggag agagattcct caagatctgg tcacaaaccg tggaggaaat
     1081 catctcctat gtcacggtca acttccctaa cccttcggga aggtctttgg aagataaatc
     1141 tacccagacc gctgggagag aactcaagaa ggaatcgcca tctgctcctc tccagagaga
     1201 aagtaaaccc ctgaaagccc gaatggaggc tcaaactgcc tctggtcctc cagctctcga
     1261 atggtctgca accaacgaag aggatgatct atctgtagag gcagaaatag cccaccagat
     1321 tgctgagagc ttttccaaga aatacaagtt cccatcccgg tcatcaggga tattcttgta
     1381 caattttgag cagttaaaaa tgaacctcga cgatatagtc aaagagtcaa aaaatgtgcc
     1441 aggtgtgacg cgtttagccc gtgaaggatc caagctccct ctaagatgtg tactgggatg
     1501 ggtcgctctg gcaaactcta agaagttcca gctattagtc gagccggaaa agctgaataa
     1561 aatcatgcag gatgatctga accgttatac atcccgctga atcgaattcc tgcagcccgg
     1621 gggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     1681 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     1741 gaggaactat atccggatcc actagttcta gagcggccgc caccgcggtg gagctccagc
     1801 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     1861 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1921 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1981 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     2041 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     2101 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     3001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     3061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3301 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3601 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3961 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
//
