LOCUS       Plasmid_CCHFV_NP      4306 bp    DNA     circular UNA 22-avr.-2011
DEFINITION  
FEATURES             Location/Qualifiers
     promoter        join(1..3,4290..4306)
                     /label="sp6 promoter"
     misc_feature    24..1594
                     /label="CCHFV_NP (U88410)"
     primer          89..107
                     /note="Oligo predicted by CLC primer designer"
                     /note="Oligo type: primer"
                     /note="Melting temp.: 55.72"
                     /label="Fwd primer"
     primer          89..107
                     /note="Oligo predicted by CLC primer designer"
                     /note="Oligo type: primer"
                     /note="Melting temp.: 55.72"
                     /label="Fwd primer"
     CDS             89..1537
                     /cds_type="ORF"
                     /note="Length: 1449"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
                     /label="CCHFV Nucleoprotein"
     misc_feature    89..118
                     /feature_type="Primer Region"
                     /note="Forward primer region"
     misc_feature    complement(1505..1534)
                     /feature_type="Primer Region"
                     /note="Reverse primer region"
     primer          complement(1516..1534)
                     /note="Oligo predicted by CLC primer designer"
                     /note="Oligo type: primer"
                     /note="Melting temp.: 55.08"
                     /label="Rev primer"
     promoter        1628..1647
                     /label="T7 promoter"
     CDS             complement(2827..3687)
                     /cds_type="ORF"
                     /note="Length: 861"
                     /note="Found at strand: negative"
                     /note="Start codon: ATG"
                     /label="AmpR"
ORIGIN
        1 GAATACGAAT TCGAGCTCGG TACCCGGGGA TCCTCTCAAA GAAACACGTG CCGCTTACGC
       61 CCACAGTGTT CTCTTGAGTG TTAGCAGAAT GGAAAACAAG ATCGAGGTGA ATAACAAAGA
      121 TGAGATGAAC AGGTGGTTTG AAGAGTTCAA AAAAGGAAAT GGACTTGTGG ACACCTTCAC
      181 AAACTCCTAT TCCTTTTGCG AGAGTGTTCC CAATTTGGAC AGGTTTGTGT TTCAGATGGC
      241 CAGTGCCACC GATGATGCAC AGAAGGACTC CATCTACGCA TCTGCTCTGG TGGAGGCAAC
      301 AAAGTTTTGT GCACCTATAT ATGAGTGCGC ATGGGTTAGC TCCACTGGCA TTGTAAAAAA
      361 GGGACTTGAA TGGTTCGAGA AAAATGCAGG AACCATTAAG TCCTGGGATG AAAGTTATAC
      421 TGAGCTAAAG GTCGACGTCC CGAAAATAGA GCAGCTTACC GGTTACCAAC AAGCTGCCTT
      481 GAAGTGGAGA AAAGACATAG GTTTCCGTGT CAATGCCAAC ACAGCAGCTC TGAGCAACAA
      541 AGTCCTCGCA GAATACAAAG TCCCTGGTGA GATTGTGATG TCTGTCAAAG AGATGCTGTC
      601 AGACATGATT AGGAGAAGGA ACCTGATTCT AAACAGGGGT GGTGATGAGA ACCCACGTGG
      661 CCCAGTGAGC CATGAGCATG TAGACTGGTG CAGGGAGTTT GTCAAAGGCA AATACATCAT
      721 GGCCTTCAAC CCACCATGGG GGGACATCAA CAAGTCAGGC CGTTCAGGAA TAGCACTTGT
      781 TGCAACAGGC CTTGCTAAGC TTGCAGAGAC TGAAGGAAAG GGAATATTTG ATGAAGCCAA
      841 AAAGACTGTG GAGGCCCTCA ACGGGTATCT GGACAAGCAT AAGGACGAAG TTGATAGAGC
      901 AAGCGCCGAC AGCATGATAA CAAACCTTCT TAAGCATATT GCCAAGGCAC AGGAGCTCTA
      961 TAAAAATTCA TCTGCACTTC GTGCACAAAG CGCACAGATT GACACTGCTT TCAGCTCATA
     1021 CTATTGGCTT TACAAGGCTG GCGTGACTCC TGAAACCTTC CCGACGGTGT CACAGTTCCT
     1081 CTTTGAGCTA GGGAAACAGC CAAGAGGTAC CAAGAAAATG AAGAAGGCTC TTCTGAGCAC
     1141 CCCAATGAAG TGGGGGAAGA AGCTTTATGA GCTCTTTGCC GATGATTCTT TCCAGCAGAA
     1201 CAGGATTTAC ATGCATCCTG CCGTGCTTAC AGCTGGTAGA ATCAGTGAAA TGGGAGTCTG
     1261 CTTTGGGACA ATCCCTGTGG CCAATCCTGA TGATGCTGCC CAAGGATCTG GACACACTAA
     1321 GTCTATTCTC AACCTCCGTA CCAACACTGA GACCAATAAT CCGTGTGCCA AAACCATCGT
     1381 CAAGCTATTT GAAGTTCAAA AAACAGGGTT CAACATTCAG GACATGGACA TAGTGGCCTC
     1441 TGAGCACTTG CTACACCAAT CCCTTGTTGG CAAGCAATCC CCATTCCAGA ACGCCTACAA
     1501 CGTCAAGGGC AATGCCACCA GTGCTAACAT CATTTAAAAT ACAAACTGCT CTGTACTCAA
     1561 CTTCCTTCCT TCTGAACCGC CATCCATAAT TGCTCTAGAG TCGACCTGCA GGCATGCAAG
     1621 CTTGTCTCCC TATAGTGAGT CGTATTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC
     1681 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG
     1741 TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC
     1801 CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG
     1861 GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
     1921 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     1981 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     2041 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     2101 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     2161 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     2221 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     2281 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2341 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2401 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2461 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     2521 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2581 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2641 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     2701 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     2761 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     2821 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     2881 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     2941 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
     3001 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     3061 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     3121 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     3181 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     3241 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     3301 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     3361 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     3421 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     3481 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
     3541 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
     3601 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
     3661 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
     3721 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
     3781 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT
     3841 CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG
     3901 TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA
     3961 AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG
     4021 GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG
     4081 TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG
     4141 GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC
     4201 GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG
     4261 ACGTTGTAAA ACGACGGCCA GTGAATTGGA TTTAGGTGAC ACTATA
//
