LOCUS       pDP049                  7009 bp    DNA     circular     18-MAR-2024
Created: Friday, May 5, 2006 10:55 AM

This plasmid contains the Tetanus toxoid C fragment (nt 164-1516, in reverse orientation!) 
WITHOUT START codon under control of the T7 promoter
The natural C-terminal STOP codon (TAA) is missing and is replaced by
Leu-Glu-His-His-His-His-His-His

Attention: the pET-28 vector contains a Kanamycin resistance gene
FEATURES             Location/Qualifiers
     misc_feature    complement(2007..2026)
                     /note="T7 promoter"
     misc_feature    complement(137..1939)
                     /note="T7-HIS-LCMV ARM NP-T7 ORF"
     misc_feature    164..1834
                     /note="LCMV ARM NP WO start and stop"
     misc_feature    5635..6450
                     /note="Kanamycin resistance "
     source          1..7009
                     /dnas_title="pDP049 pET-28-TTC-HIS"
ORIGIN      
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagGAGTGTC ACAACATTTG
      181 GGCCTCTAAA AATTAGGTCA TGTGGCAGAA TGTTGTGAAC AGTTTTCAGA TCTGGGAGCC
      241 TTGCTTTGGA GGCGCTTTCA AAAATGATGC AGTCCATGAG TGCACAGTGC GGGGTGATCT
      301 CTTTCTTCTT TTTGTCCCTT ACTATTCCAG TATGCATCTT ACACAACCAG CCATATTTGT
      361 CCCACACTTT gTCTTCATAC TCCCTCGAAG CTTCCCTGGT CATTTCAACA TCGATAAGCT
      421 TAATGTCCTT CCTATTtTGT GAGTCCAGAA GCTTTCTGAT GTCATCGGAG CCTTGACAGC
      481 TTAGAACCAT CCCCTGCGGA AGAGCACCTA TAACTGACGA GGTCAACCCG GGTTGCGCAT
      541 TGAAGAGGTC GGCAAGATCC ATGCCGTGTG AGTACTTGGA ATCTTGCTTG AATTGTTTTT
      601 GATCAACGGG TTCCCTGTAA AAGTGTATGA ACTGCCCGTT CTGTGGTTGG AAAATTGCTA
      661 TTTCCACTGG ATCATTAAAT CTACCCTCAA TGTCAATCCA TGTAGGAGCG TTGGGGTCAA
      721 TTCCTCCCAT GAGGTCTTTT AAAAGCATTG TCTGGCTGTA GCTTAAGCCC ACCTGAGGTG
      781 GACCTGCTGC TCCAGGCGCT GGCCTGGGTG AgTTGACTGC AGGTTTCTCG CTTGTGAGAT
      841 CAATTGTTGT GTTTTCCCAT GCTCTCCCCA CAATCGATGT TCTACAAGCT ATGTATGGCC
      901 ATCCTTCACC TGAAAGGCAA ACTTTATAGA GGATGTTTTC ATAAGGGTTC CTGTCCCCAA
      961 CTTGGTCTGA AACAAACATG TTGAGTTTTC TCTTGGCCCC GAGAACTGCC TTCAAGAGgT
     1021 CCTCGCTGTT GCTTGGCTTG ATCAAAATTG ACTCTAACAT GTTACCCCCA TCCAACAGGG
     1081 CTGCCCCTGC CTTCACGGCA GCACCAAGAC TAAAGTTATA GCCAGAAATG TTGATGCTGG
     1141 ACTGCTGTTC AGTGATGACC CCCAGAACTG GGTGCTTGTC TTTCAGCCTT TCAAGATCAT
     1201 TAAGATTTGG ATACTTGACT GTGTAAAGCA AGCCAAGGTC TGTGAGCGCT TGTACAACGT
     1261 CATTGAGCGG AGTCTGTGAC TGTTTGGCCA TACAAGCCAT AGTTAGACTT GGCATTGTGC
     1321 CAAATTGATT GTTCAAAAGT GATGAGTCTT TCACATCCCA AACTCTTACC ACACCACTTG
     1381 CACCCTGCTG AGGCTTTCTC ATCCCAACTA TCTGTAGGAT CTGAGATCTT TGGTCTAGTT
     1441 GCTGTGTTGT TAAGTTCCCC ATATATACCC CTGAAGCCTG GGGCCTTTCA GACCTCATGA
     1501 TCTTGGCCTT CAGCTTCTCA AGGTCAGCCG CAAGAGACAT CAGTTCTTCT GCACTGAGCC
     1561 TCCCCACTTT CAAAACATTC TTCTTTGATG TTGACTTTAA ATCCACAAGA GAATGTACAG
     1621 TCTGGTTGAG ACTTCTGAGT CTCTGTAGGT CTTTGTCATC TCTCTTTTCC TTCCTCATGA
     1681 TCCTCTGAAC ATTGCTGACC TCAGAGAAGT CCAACCCATT CAGAAGGTTG GTTGCATCCT
     1741 TAATGACAGC AGCCTTCACA TCTGATGTGA AGCTCTGCAA TTCTCTTCTC AATGCTTGCG
     1801 TCCATTGGAA GCTCTTAACT TCCTTAGACA AGGAAGAgga tccgcgaccc atttgctgtc
     1861 caccagtcat gctagccata tggctgccgc gcggcaccag gccgctgctg tgatgatgat
     1921 gatgatggct gctgcccatg gtatatctcc ttcttaaagt taaacaaaat tatttctaga
     1981 ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg
     2041 agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     2101 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     2161 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     2221 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     2281 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagat cccggacacc
     2341 atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc
     2401 agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
     2461 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
     2521 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
     2581 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
     2641 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
     2701 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
     2761 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
     2821 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
     2881 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
     2941 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
     3001 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
     3061 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
     3121 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
     3181 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
     3241 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
     3301 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     3361 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     3421 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     3481 gaaagcgggc agtgagcgca acgcaattaa tgtaagttag ctcactcatt aggcaccggg
     3541 atctcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     3601 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     3661 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     3721 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     3781 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccccacg
     3841 ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
     3901 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
     3961 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
     4021 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     4081 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
     4141 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
     4201 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
     4261 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
     4321 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
     4381 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
     4441 tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4501 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     4561 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     4621 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     4681 caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     4741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5341 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5581 tggtcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     5641 catattcaac gggaaacgtc ttgctctagg ccgcgattaa attccaacat ggatgctgat
     5701 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga
     5761 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     5821 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     5881 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     5941 gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     6001 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     6061 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     6121 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     6181 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     6241 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     6301 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     6361 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     6421 tttcatttga tgctcgatga gtttttctaa gaattaattc atgagcggat acatatttga
     6481 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6541 tgaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6601 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6661 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6721 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6781 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     6841 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     6901 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     6961 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca
//

