EVAg

The 3' terminal 217 nts of Aeonium ringspot virus RNA1 and RNA2 (excluding the poly(A) tail) are cloned in pGEM-T Easy allowing for transcription of a riboprobe to detect both genomic RNAs.

The full-length genome of CpCDV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants

The full-length genome of TYLCSV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants

Double-stranded DNA copy of a coxsackievirus A17 genome without the capsid-encoding region, cloned into a plasmid backbone downstream a T7 promoter. After in vitro transcription, the corresponding RNA can be transfected into cells to give rise to a replicon that is self-replicative and can express the non-structural virus proteins but is unable to produce infectious particles. The plasmid can be amplified in E. coli.