EVAg

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

We would like to invite African researchers to a training workshop on virus diagnostics. In the scope of this three-day workshop, participants from various African countries will acquire hands-on experience in the detection and characterisation of viruses using electron microscopy and Reverse Transcription Polymerase Chain Reaction (RT-PCR). This workshop offers a valuable opportunity for participants to connect and to network with each other, contributing to the prevention of virus outbreaks.

The detection of plant viruses in environmental samples, such as insects, mites, crops, weeds. obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology. The service offers the individual analysis of three samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

This service is intended for a global analysis of viral reference samples in order to obtain information about the genomic sequences on the one hand, and on the other hand, it opens the possibility to check the purity at the same time in order to exclude possible contaminations. It is used for quality control and certification of reference material.

The detection of viral pathogens infecting a given plant sample, obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology or for the certification of seed and planting material. The service offers the individual analysis of three plant samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

Specific tests for certain regulated viruses are required for the certification of seed and planting material. However, the main limitation of this approach is that only these viruses are detected. Latent and/or unknown viruses, which can also have a significant impact on yield and product quality, remain unidentified. Especially in vegetative propagation material, this inevitably leads to the accumulation of such pathogens. High-throughput sequencing and bioinformatics analysis offers a global analysis of the phytosanitary status of plants.

In this two-week course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus typing and molecular characterization will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: sample preparation, nucleic acid extraction, rolling-circle amplification, RFLP analysis, cloning, sequencing, phylogenetic analysis.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting a plant or fungal sample, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed

In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens.

Access to AMU/AFMB infrastructure for learning/training on techniques related to viral recombinant proteins