A/swine/Italy/150383-1/2014 (H1N2)


EVAg

Originator
:
https://www.izsler.it/
Produced by
: IZSLER
Shipping From
: Brescia - IT

Product Description

Ref-SKU:
026V-04380
The virus has been isolated from swine and propagated on Caco2 and MDCK cell line
Product Risk Group: 
Genotype: 
Serotype: 

Storage conditions: 
Freeze Dried
Sequencing: 
Complete genome
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Not tested

Unit definition: 
1 mL

In stock

550,00 €
(Cost per access for Academics)

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GMO: 
No
Biosafety restrictions: 
Human Risk Group 1, Animal Risk Group 2
Virus host type: 
Infectivity Test: 
HA test
Viral titer: 
HA titer 1:64 (MDCK), >128 (CACO2)
Production cell line: 
Genbank reference: 
Virus Cultivability: 
Passage: 
1 x Caco2, 1 x MDCK
Identification technique: 
PCR, sequencing
Technical recommendation: 
PCR, sequencing
Shipping conditions: 
IATA Classification: 

Information about the collection of the virus

Recombinant product: 
No
Biological material origin: 
Natural origin
Collection date: 
Wednesday, 11 June, 2014
Country of collection: 
Suspected epidemiological origin : 
Isolation host: 
Isolation technique: 
Propagation on Caco2 , propagation on MDCK cell line
Isolation conditions: 
CACO-2 cells were cultured in 25 cm2 tissue culture flask in MEM with 1 mM of sodium pyruvate and 15% foetal calf serum. When the cells formed a monolayer, usually after 48 h, the medium was removed and cells were washed three times with saline solution. Each sample was inoculated onto CACO-2 cells (1ml) followed by 1ml of MEM with 1 mM of sodium pyruvate. The inoculum was allowed to adsorb at 37 °C for 45 min in presence of 5% CO2 and then 8 ml of MEM with 1 mM of sodium pyruvate was added to all wells. MDCK cells were cultured in 25 cm2 tissue culture flask in Eagle's minimum essential medium in Earle's balanced salt solution (MEM) with 10% foetal calf serum . When the cells formed a monolayer, usually after 48 h, the medium was removed and the cells were washed three times with saline solution. Each sample was inoculated onto MDCK cells (1 ml) followed by 1 ml of MEM containing 10 μg/ml of trypsin. The inoculum was allowed to adsorb at 37 °C for 45 min in presence of 5% CO2 and then 8 ml of MEM with 1 μg/ml of trypsin was added