Human 2019-nCoV strain 2019-nCoV/Italy-INMI1


EVAg

Produced by
: INMI
Shipping From
: Roma - IT

Product Description

Ref-SKU:
008V-03893
Infectious cell culture supernatant containing Human 2019-nCoV strain 2019-nCoV/Italy-INMI1. This strain is preserved under Viral Storage Medium -80C. To confirm its identity the virus has been completely sequenced. The complete sequence was submitted to GenBank (SARS-CoV-2/INMI1-Isolate/2020/Italy: MT066156) and is available on GISAID website (BetaCoV/Italy/INMI1-isl/2020: EPI_ISL_410545) upon registration. This strain is preserved under Viral Storage Medium -80C and is Mycoplasma and FMDV free. Import permit can be needed in your country. If yes, please indicate it in your online enquiry.
Product Risk Group: 
Virus name: 
Strain: 
2019-nCoV/Italy-INMI1

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Complete genome
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 vial of 0.5 ml

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Infectivity Test: 
Limiting dilution assay, by determining tissue culture infectious dose (TCID50) with the method of Reed & Muench
Viral titer: 
10^7.25 TCID50/ml
Production cell line: 
Geographical origin: 
Collection date: 
Wednesday, 29 January, 2020
Isolation host: 
Isolation technique: 
cell culture
Passage: 
2P
Identification technique: 
PCR and sequencing
Technical recommendation: 
Culture conditions: 37°C and 5% CO2. Culture Medium: Minimum Essential Medium Eagle supplemented with 2% heat-inactivated FBS and 1% L-Glutamine.
Shipping conditions: 
IATA Classification: 










Comments (3)

  • BN264's picture

    Could I get the inactivated virus?
    I want to know whether I can receive it as it is inactivated.

    Feb 12, 2020
  • S.Meschi's picture

    Dear Dr Lee,

    wich type of the inactivation do you need?

    We can provide non infectious cell culture supernatant containing Human 2019-nCoV strain 2019-nCoV/Italy-INMI1 RNA in the AVL lysis buffer for the implementation of molecular tests.

     

    Otherwise we can provide non infectious cell culture supernatant containing Human 2019-nCoV strain 2019-nCoV/Italy-INMI1 treated with Triton X-100 for the implementation of serological tests. The chemical inactivation can disrupt the lipid membrane of the virus without affecting proteins.

    Feb 13, 2020
  • tannerma1's picture

    Hello S. Meschi,

    I would be interested to learn how to access inactivated Human 2019-nCoV strain 2019-nCoV/Italy-INMI1 Virus. Would I put in an enquiry order for the live virus and then after a reply ask about getting the virus inactivated?

    Thanks very much,

    Dr. Michael Tanner
    michael.tanner@thermofisher.com

    Feb 22, 2020