Human 2019-nCoV strain 2019-nCoV/Italy-INMI1
Infectious cell culture supernatant containing Human 2019-nCoV strain 2019-nCoV/Italy-INMI1. This strain is preserved under Viral Storage Medium -80C. To confirm its identity the virus has been completely sequenced. The complete sequence was submitted to GenBank (SARS-CoV-2/INMI1-Isolate/2020/Italy: MT066156) and is available on GISAID website (BetaCoV/Italy/INMI1-isl/2020: EPI_ISL_410545) upon registration. This strain is preserved under Viral Storage Medium -80C and is Mycoplasma and FMDV free. Import permit can be needed in your country. If yes, please indicate it in your online enquiry.
Product Risk Group:
Viral Storage Medium -80C
Infectivity tested and quantified
Limiting dilution assay, by determining tissue culture infectious dose (TCID50) with the method of Reed & Muench
Production cell line:
PCR and sequencing
Culture conditions: 37°C and 5% CO2. Culture Medium: Minimum Essential Medium Eagle supplemented with 2% heat-inactivated FBS and 1% L-Glutamine.
For virus stock preparation, Vero E6 cells were infected. Cell lysates, obtained through freezing/defreezing cycles, were clarified and aliquoted.