Hyperimmune polyclonal antiserum raised in a domestic pig challenged with Japanese encephalitis virus (JEV) Nakayama strain and boosted 47 days later. Serum was collected at day 83 post primary challenge.
Carnation Italian ringspot virus full-length cDNA cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNA
Unit: 2 micrograms plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
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