EVAg

Hybridoma cell culture supernatant containing anti-Influenza A virus antibodies; targeted antigen: Matrix Protein 1; clone: 4.2.1.A; immunization antigen: several inactivated AIV HxN2 isolates

Plasmid for expression of the LCMV Armstrong strain L-segment in rodent cells; [also termed pI-L-ARM(-) or pDP067]

Expression plasmid of the LCMV strain clone 13 "S" segment expressing WE glycoprotein [also termed pol-I-S-WE(-) or pDP009]

Plasmid for the expression of the LCMV strain Armstrong L-protein in eukaryotic cells. [also termed pC-L_ARM-BsmB pDP062]

Expression plasmid of the LCMV strain Armstrong S segment in rodent cells [also termed polI-S-ARM or pDP073]

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

Recombinant 3C protease of Coxsackie B3 virus. Produced in E. coli, purified in non denaturing conditions. Suitable for protein crystallization, functional assays, or tag cleavage.

Hybridoma cell culture supernatant containing anti-CCHFV antibodies; targeted antigen: glykoprotein Gn/Gc; clone: 4H12B3; immunization antigen: recombinant Gn/Gc protein, strain "IbAr 10200"

Hybridoma cell culture supernatant containing anti-SPDV antibodies; targeted antigen: unknown; clone: 15B8; immunization antigen: inactivated strain "S49P (rainbow trout)"

Hybridoma cell culture supernatant containing anti-SPDV antibodies; targeted antigen: envelope; clone: 12A5; immunization antigen: inactivated strain "S49P (rainbow trout)"

DNA extracted from clarified supernatant from infected cell culture.

DNA extracted from clarified supernatant from infected cell culture.

DNA extracted from clarified supernatant from infected cell culture.

In this 1 week course the basic principles of methods used to conduct phylogenetic analysis of enterovirus genomes will be addressed by a combination of lectures, demonstration and hands-on training. Participants will perform all required analysis procedures using different bioinformatics tools. Topics included: Selection of genomic regions used for phylogenetic analysis – Selection of relevant sequences in public database to be included in the analysis – Search for recombination events

This course constitutes an optional week that can supplement the course « Isolation of enteroviruses from clinical samples – Detection by real-time RT-PCR ». This training will focus on methods used to perform titration of enterovirus stock solutions and to conduct enterovirus purification by plaque assays.

In this 3 weeks course the basic principles of routine enterovirus isolation on cell lines will be addressed by a combination of lectures, demonstration and hands-on training. Participants will perform all required laboratory and analysis procedures for routine isolation of enterovirus. Topics included: Presentation and handling of different cell lines suitable for enterovirus isolation – Inoculation of cells with enterovirus samples in a BSL-2 facility - Nucleic acid extraction and real-time RT-PCR for enterovirus detection.

Institut Pasteur Paris - In this 3-week course, the basic principles of methods used to generate full-length genomic sequences of enteroviruses by next-generation sequencing will be addressed by a combination of lectures, demonstration and hands-on training. Participants will perform all required laboratory and analysis procedures (except the preparation of the Illumina libraries that is performed by an external contractor).

Institut Pasteur Paris - In this 3 weeks course the basic principles of routine enterovirus molecular typing will be addressed by a combination of lectures, demonstration and hands-on training. Participants will perform all required laboratory and analysis procedures for routine typing of enterovirus. Topics included: sample preparation, nucleic acid extraction - reverse transcription and PCR - assay validation and quality control - Sequencing and sequence analysis - Basic phylogeny for virus typing.

Expression clone for the SAD virus matrix protein from SAD Rabies Virus

Expression clone for the SAD virus matrix protein in fusion with a 3XFlag tag encoding sequence at its 5'.

recombinant Aichivirus protease expressed in E. coli and purified under native conditions. The protein was expressed with a Cternminal 6his tag.

Through direct cloning and expression of the variable heavy chains (VHHs) of HCAbs from the bone marrow of MERS-CoV infected dromedaries, MERS-CoV specific VHHs or nanobodies were produced. VHH-83 binds with exceptionally high affinity to the receptor binding domain of the viral spike protein. In vitro, this VHH efficiently blocks virus entry at picomolar concentrations

HCAb-83 is a MERS-CoV specific camel/human chimeric heavy chain only antibody (HCAb) - composed of the camel VHH-83 linked to a human Fc domain lacking the CH1 exon Through direct cloning and expression of the variable heavy chains (VHHs) of HCAbs from the bone marrow of MERS-CoV infected dromedaries, MERS-CoV specific VHHs or nanobodies were produced. VHH-83 binds with exceptionally high affinity to the receptor binding domain of the viral spike protein. In vitro, HCAb-83 efficiently blocks virus entry at picomolar concentrations

Cowpox virus isolated from cat, strain CatBer 07/1, genomic DNA from viral cell culture supernatant (Hep-2 cells)