Live Porcine Circovirus, PCV-1, isolate 2008-0154. This Circovirus[2008-0154] is preserved under Viral Storage Medium -80C. Tests for the presence of mycoplasmae were not performed.
Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Slovenia/LP3/46/23; lineage Omicron, FL.1.5.1). This strain is preserved in viral storage medium -80°C.
Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions.
The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection
Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.
Unit: 2 micrograms of each plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription
