Lyophilized ready-to-use primers and probe (Lyoph-P&P, 24 rxns in the form of 3x8 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) in the RdRp gene.
Primers and Probe sequences designed by Charité Universitätsmedizin Berlin. https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf.
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase
For RUO (research use only)
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) in the RdRp (IP4) gene.
Primers and Probe sequences designed by Institut Pasteur: https://www.who.int/docs/default-source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-pasteur-paris.pdf?sfvrsn=3662fcb6_2)
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase
For RUO (research use only)
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of SARS-CoV-2 (2019-nCoV) in the N (CUB) gene.
Primers and Probe sequences designed by Charité Universitätsmedizin Berlin; https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf).
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase
For RUO (research use only)
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 2x48 rxns) for real time RT-PCR in a region of the MS2 phage for internal control.
Disclaimer: This product does not contain the polymerase
For RUO (research use only)
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 45 rxns in the form of 5x9 rxns) for real time PCR of Monkeypox virus (F3L gene).
Primers and Probe sequences. Reference article : Kulesh D.A. et al. 2004: Monkeypox virus detection in rodents using real-time 3'-minor groove binder TaqMan assays on the Roche LightCycler.
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Note: Expected delay due to dual use regulation (export permit necessary)
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 45 rxns in the form of 5x9 rxns) for real time PCR of Orthopoxviruses (OPE9L gene).
Primers and Probe sequences : Reference article : Kulesh D.A. et al. 2003:Smallpox and pan-Orthopox Virus Detection by Real-Time 3-Minor Groove Binder TaqMan Assays on the Roche LightCycler and the Cepheid Smart Cycler Platforms.
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Note: Expected delay due to dual use regulation (export permit necessary)
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 12x8 rxns) for real time PCR of Sudan virus (Ebolavirus)
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Extracted RNA of Sudan ebolavirus Gulu can be found here : Ref-SKU: 002N-04805
https://www.european-virus-archive.com/nucleic-acid/sudan-ebolavirus-strain-gulu-rna
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 4x24 rxns) for real time RT-PCR of Marburg virus
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 12x8 rxns) for real time PCR of Ebolavirus Zaïre
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 24x4 rxns) for real time PCR of Severe fever with thrombocytopenia syndrome virus
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 24x4 rxns) for real time RT-PCR of Sindbis virus
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 24x4 rxns) for real time RT-PCR of Zika virus (Taqman)
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 24x4 rxns) for real time RT-PCR of chikungunya virus (Taqman)
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 24x4 rxns) for real time RT-PCR of dengue virus (all serotypes) (Taqman)
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Lyophilized ready-to-use primers and probe (Lyoph-P&P, 96 rxns in the form of 24x4 rxns) for real time RT-PCR of West Nile virus (Taqman)
SOPs in the attached pdf.
Disclaimer: This product does not contain the polymerase.
For RUO (research use only).
Training Description: Bioinformatics for the design of real-time detection systems. Production of positive control. Extraction of nucleic acid. Real time RT-PCR and data analysis. Validation of the training with a case study performed in autonomy.
Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions.
The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection
Production on request of stabilisated RNA positif control, as known as Armored RNA for RT-PCR. This service includes:
- Design of the positif control sequence based on the sequence provided by the costumer
- Production and lyophilisation of Armored RNA according the quantity needed
- LOD/quantification
- Shipment of the Armored RNA at room temperature