full length plasmid clone of proviral HIV-1; construct produces fully infectious HIV-1 upon transfection into eukaryotic cells; most of chromosomal flank DNA removed
Aeonium ringspot virus, RNA1 and RNA2 full-length cDNAs are cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNAs.
Unit: 2 micrograms of each plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription