The full-length genome of TYLCSV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants
Unit: 1 microgram, allowing infection of plants following transformation into Agrobacterium
The 3' terminal 217 nts of Aeonium ringspot virus RNA1 and RNA2 (excluding the poly(A) tail) are cloned in pGEM-T Easy allowing for transcription of a riboprobe to detect both genomic RNAs.
Unit: 2 micrograms of plasmid DNA, allowing for the synthesis of a probe after T7-driven transcription
The full-length genome of TYLCV (1.7 copies) is cloned in the binary plasmid vector pCambia 0380, allowing transformation into Agrobacterium and infection of plants.
The full-length genome of TYLCV-Mld (2 copies in tandem) is cloned in the binary plasmid vector pCambia 2300 allowing transformation into Agrobacterium and infection of plants
The full-length genome of PYMV-A (1.44 copies) is cloned in the binary plasmid vector pCambia 0380 allowing transformation into Agrobacterium and infection of plants
The full-length genome of PYMV-B component (1.54 copies) is cloned in the binary plasmid vector pCambia 0380, allowing transformation into Agrobacterium and infection of plants
The full-length genome of Tomato leaf curl Comoros virus(1.2 copies) is cloned in the binary plasmid vector pCambia 2300, allowing transformation into Agrobacterium and infection of plants
The full-length genome of CpCDV (1.8 copies) is cloned in the binary plasmid vector pBin19, allowing transformation into Agrobacterium and infection of plants
Unit: 1 microgram, allowing infection of plants following transformation into Agrobacterium
