Product Description
Ref-SKU:
006N-02575 HIV-1, drug resistant point mutant of protease, selected by specific HIV protease inhibitors.
Product Risk Group:
Information on the related virus
ICTV Taxonomy:
Virus name:
Subspecies:
Strain:
Cloned Product: Yes
Can it be used to produce GMO:
No
Biosafety restrictions:
May reconstitute infectious virus after transfection into suitable human cells
Region encompassed in this Product:
Full length viral clone
Plasmid:
Plasmid selection:
TAG:
Sequencing:
Fully sequenced
Sequence checked:
Yes
Mutations:
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations:
The virus genome is derived from pNL-NF (pNL4-3 with shortened cellular flank) (doi.org/10.1016/j.jcv.2006.05.001).
mutations as indicated in the name; backbone is NL-BX.
A unique BamHI site was introduced 5’ of protease (ggagcc2207ggaTcc; A->S in Pol; E->D in Gag) and an XmaI site 3’ of protease (ccagga2589ccCggG).
The original BamHI site as position 8465 was removed (GGATCC->GGATCA change silent in Env; L->S in Rev).
Other mutations as indicated in the product name.
Storage conditions:
TE Buffer -20C
Shipping conditions:
IATA Classification:
Virus host type:
Special feature:
Note:
To identify the resistance(s) to antiretroviral drugs caused by the mutations present in this viral clone, use e.g. the „mutations list analysis“ function of the public HIV-Grade site at:
Information about the origin of the product
Recombinant product:
This product is a recombinant product
Biological material origin:
Synthetic origin & Natural origin
Natural part identification: NL4-3
Natural part identification:
NL4-3
Genbank reference:
Collection date: BEFORE
Wednesday, 1 September, 2010
Country of collection:
Synthetic part identification: point mutation: Val at position 84 in the protease gene
Synthetic part identification:
point mutation: Val at position 84 in the protease gene
Modifications from the reference sequence(s):
The sequence(s) of this synthetic product has been modified from the sequence listing provided as Genbank references
Description of modification(s) made from the reference sequence(s):
PCR-based mutagenesis; carries the indicated mutation(s) in the target gene as shown in the product name