Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Slovenia/LP3/46/23; lineage Omicron, FL.1.5.1). This strain is preserved in viral storage medium -80°C.
The 3' terminal 217 nts of Aeonium ringspot virus RNA1 and RNA2 (excluding the poly(A) tail) are cloned in pGEM-T Easy allowing for transcription of a riboprobe to detect both genomic RNAs.
Unit: 2 micrograms of plasmid DNA, allowing for the synthesis of a probe after T7-driven transcription