Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions.
The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection
Freeze-dried Japanese encephalitis virus, strain UVE/JEV/UNK/TW/RP9-190, Taiwan, complete ORF sequence. It was produced by ISA method from Infectious clone RP-9 (PMID:8873412)
