EVAg

Virus Neutralization Tests (VNT), also including plaque-reduction and foci-inhibition assays like PRNT and RFFIT, are the gold standard for determining antibody titers against viruses, and are essential serological tools. In this 5-day training the application and basic principles of VNTs will be discussed, and will be combined with wet lab training. The wet lab training will include all steps of the VNT, including analysis and interpretation of the results.

The detection of viral pathogens infecting a given plant sample, obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology or for the certification of seed and planting material. The service offers the individual analysis of three plant samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

Development and production of Diagnostic systems with positive control for arboviruses (RUO)

Production on request of stabilisated RNA positif control, as known as Armored RNA for RT-PCR. This service includes: - Design of the positif control sequence based on the sequence provided by the costumer - Production and lyophilisation of Armored RNA according the quantity needed - LOD/quantification - Shipment of the Armored RNA at room temperature

The service aims at offering the possibility to evaluate the outcome of an infection by a SARS-CoV-2 strain or variant in a susceptible cell line : evaluation of the susceptibility of a cell line, evaluation of the infectivity of a variant compared to a reference variant, evaluation of the effect of infection on a cellular pathway etc... The project can be refined upon request

Specific tests for certain regulated viruses are required for the certification of seed and planting material. However, the main limitation of this approach is that only these viruses are detected. Latent and/or unknown viruses, which can also have a significant impact on yield and product quality, remain unidentified. Especially in vegetative propagation material, this inevitably leads to the accumulation of such pathogens. High-throughput sequencing and bioinformatics analysis offers a global analysis of the phytosanitary status of plants.

Generation of recombinant chimeric monoclonal antibodies: murine monoclonal antibodies (hybridoma cells or total RNA have to be provided by the end-user) will be sequenced, cloned, recombinantly expressed in Expi293 cells, and purified via protein G. Available backbones for heavy and light chain constant regions are: mouse IgG1, Ig kappa; mouse IgG2a, Ig kappa; rabbit IgG, Ig kappa; human IgG1, Ig kappa; porcine IgG, Ig kappa; and bovine IgG, Ig kappa. Other backbones could possibly also generated uppon request.

In this two-week course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus typing and molecular characterization will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: sample preparation, nucleic acid extraction, rolling-circle amplification, RFLP analysis, cloning, sequencing, phylogenetic analysis.

In this five-day course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus diagnosis will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: tissue-printing hybridization, dot-blot hybridization, preparation of non-radioactive probes, rapid sample extraction. After this course participants should have sufficient knowledge to introduce routine diagnostics of geminiviruses in their own laboratory.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting a plant or fungal sample, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed

In an outbreak situation in crops, it is necessary to obtain quickly a good overview of the viruses that may be involved. Only in this way can the right measures be taken to safeguard crop production immediately but also in the long term. High-throughput sequencing (HTS) and bioinformatics analysis of samples offer a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens.

High-throughput sequencing and bioinformatics analysis of samples for a global analysis of the phytosanitary status of plants and the rapid and precise identification of pathogens. Can be used for the discovery of novel or rare virus variants, the characterization of the genetic variation and the population composition.

Access to AMU/AFMB infrastructure for learning/training on techniques related to viral recombinant proteins

Training in Coronavirus reverse genetics to adapt novel techniques (ISA method) to large RNA viruses and compare different technologies under BSL-3 conditions

SARS-CoV-2 diagnostic training, including differential diagnostics of human Coronaviruses and other respiratory pathogens, incl. BSL-2 and BLS-3 conditions.

RG2 virus production (available on the EVAg catalogue) in single batch upon request containing 30 freeze-dried vials. The specific detection system (5 vials) by real-time PCR will be provided in the same package.

Institut Pasteur Paris - Access to BSL 3 containment facility. The access will comprise a detailed theoretical and subject to availability practical application of the biosafety and biosecurity management at Institut Pasteur. Among others, establishment and validation of suitable decontamination methods for waist, rooms and material, as well as documentation and information management will be addressed.

In this 3-day course the basic principles of the protein microarray will be addressed by a combination of lectures, demonstration and hands-on training. The protein microarray technique can be used for HA antibody profiling for group level assessment of exposures to influenza viruses in small sample volumes from humans and animals (birds, horses, pigs, bats, carnivores a.o.). The training encompasses wet lab training but also the analysis and interpretation of assay results. Special attention will be given to specific issues of Influenza and flavivirus cross reactivity.

Diagnostic training in zooonotic viruses under BSL-2/BSL-3 conditions at the Institute of Virology - Charité Universitätsmedizin Berlin, Germany.

Comparative arbovirus diagnostics training, including molecular and serologic techniques under BSL-2 and BSL-3 conditions

Kidney cells (epithelial)/ fibroblast–like, origin: Chlorocebus sabaeus (African green monkey); CRISPR/Cas9-engineered IFN-Lambda deficient phenotype

The generation of an antigen-specific murine monoclonal hybridoma cell line against a specific antigen provided by the end-user (purified Protein or inactivated purified virus) included: 1. the immunization of 2 BALB/c mice, 2. the fusion of the spleen cells with SP2/0 cells, 3. Screening and selection of suitable clones (~ 10 to 15) by using a suitable screening method depending of the planned later usage, 4.

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

This five-day course includes two modules that can be attended separately. In the first module (Ref-SKU: 011S-02851), the basic principles of routine virus typing that can be used for hospital epidemiology and public health will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for routine typing of viruses.

In this 3-day course the basic principles of serological testing against ARBO viruses will be addressed by a combination of lectures, demonstration and hands-on training. Participants will visit the Viroscience clinical virology lab, where they will perform all laboratory techniques including ELISA, Immunofluorescence and virus neutralization. Attention will be given to serological testing in cerebrospinal fluid, including the calculation of an antibody index.