EVAg

During visit of the facility examples for comprehensive detection and measures to control quarantine pathogens (viruses, bacteria, fungi, nematodes) will be presented and the associated quality management will be demonstrated. Furthermore time for mutual scientific exchange and discussions on the control of known and emerging viruses is given. The number of applicants is limited to one to two persons per visiting period.

High-throughput sequencing has developed into a versatile standard tool in plant virology research. It provides fast and precise genomic data on the viruses being processed for a wide range of applied and basic research questions. This service package offers the individual analysis of three plant samples by HTS to ensure a reliable detection and assembly of the viral genomes.

Course instruction will be conducted in the high containment facility at ACDP, Geelong, Victoria, Australia. Participants will receive a 5-day intensive course that includes interactive theoretical and practical experiences. Topics addressed will include introduction to BSL4 suited laboratories, regulatory frameworks, operational oversight, engineering controls and maintenance considerations.

An in-depth theoretical and practical application of biosafety, biocontainment and biosecurity under the Australia/New Zealand standards and Australian regulatory framework for Biosecurity Containment and Physical Containment level 3 and 4 laboratory and animal facilities.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect castor bean tick nymphs (Ixodes ricinus). The nymphs will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 nymphs will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 nymphs and provided to the end-user.

The end-user provided virus (e.g. as a tick homogenate) will be used to experimentally infect adult castor bean ticks (Ixodes ricinus). The ticks will be infected by micro-capillary inoculation into the coxal plate of the second pair of legs (transcoxal infection) using a digital microinjector system. First, 100 ticks will be used for evaluation of the virus replication dynamics. Based on the obtained results, tick-derived virus stock will be generated from an additional 100 ticks and provided to the end-user.

Virus production of viruses available on the EVAg catalogue from BMC-SAS in a single batch upon request containing 30 cryotubes containing 0.5mL.

Virus production of viruses available on the EVAg catalogue from BMC-SAS in a single batch upon request containing 30 cryotubes containing 0.5mL.

Human Immunodeficiency Virus (HIV) drug resistance remains a significant health issue that challenges treatment and prevention. The objective of this 3-week course is to learn bioinformatics techniques for the analysis of next-generation HIV sequencing results. This covers both HIV-1 and HIV-2. Teaching objectives include data curation, alignment, phylogenetics for typing, identification of drug resistance mutations, production of a resistance score, and interpretation. This course does not include genotyping itself, only sequence analysis and interpretation, as described above.

We offer a 5 day training session to colleagues from endemic regions. Participants will visit the Viroscience clinical virology lab, where they will perform all required laboratory and analysis procedures for the quantification rabies virus and neutralizing antibodies.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

We would like to invite African researchers to a training workshop on virus diagnostics. In the scope of this three-day workshop, participants from various African countries will acquire hands-on experience in the detection and characterisation of viruses using electron microscopy and Reverse Transcription Polymerase Chain Reaction (RT-PCR). This workshop offers a valuable opportunity for participants to connect and to network with each other, contributing to the prevention of virus outbreaks.

The detection of plant viruses in environmental samples, such as insects, mites, crops, weeds. obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology. The service offers the individual analysis of three samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

This four day course will go through the general principles for the diagnosis of bovine leukemia virus, through a combination of discussions, demonstrations and hands-on training. The training will include sample processing, PCR, ELISA (serology), reagent production, virus related bio-safety and diagnostic quality systems.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting plant samples, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed.

Access to RIVM infrastructure to use anonymized human fecal material containing fully sequenced norovirus strains, representing different genotypes. Strains/genotypes/year (2012-2023) selection on demand. Custom-made package includes individual fecal material from 5 different patients, 10-20% suspended in virus culture medium. Service includes technical input on protocols and experiment design and support in the laboratory. Individual questions to refine potential requests can be sent to RIVM-EVAglobal@rivm.nl.

Access to RIVM infrastructure to use anonymized human fecal material containing fully sequenced sapovirus strains, representing different genotypes. Strains/genotypes/year (2012-2023) selection on demand. Custom-made package includes individual fecal material from 2 different patients, 10-20% suspended in virus culture medium. Service includes technical input on protocols and experiment design and support in the laboratory. Individual questions to refine potential requests can be sent to RIVM-EVAglobal@rivm.nl.

Virus Neutralization Tests (VNT), also including plaque-reduction and foci-inhibition assays like PRNT and RFFIT, are the gold standard for determining antibody titers against viruses, and are essential serological tools. In this 5-day training the application and basic principles of VNTs will be discussed, and will be combined with wet lab training. The wet lab training will include all steps of the VNT, including analysis and interpretation of the results.

The detection of viral pathogens infecting a given plant sample, obtained through high-throughput sequencing (HTS) followed by appropriate bioinformatics analyses, allows to verify the phytosanitary status of plants and is particularly useful for the identification of viruses involved in diseases with unknown etiology or for the certification of seed and planting material. The service offers the individual analysis of three plant samples by HTS and the downstream bioinformatic analysis, up to the identification of sequences of viral origin in each sample.

Generation of recombinant chimeric monoclonal antibodies: murine monoclonal antibodies (hybridoma cells or total RNA have to be provided by the end-user) will be sequenced, cloned, recombinantly expressed in Expi293 cells, and purified via protein G. Available backbones for heavy and light chain constant regions are: mouse IgG1, Ig kappa; mouse IgG2a, Ig kappa; rabbit IgG, Ig kappa; human IgG1, Ig kappa; porcine IgG, Ig kappa; and bovine IgG, Ig kappa. Other backbones could possibly also generated uppon request.

In this two-week course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus typing and molecular characterization will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: sample preparation, nucleic acid extraction, rolling-circle amplification, RFLP analysis, cloning, sequencing, phylogenetic analysis.

In this five-day course, to be held in the CNR-IPSP laboratories in Torino (Italy), the basic principles of geminivirus diagnosis will be addressed by a combination of lectures, demonstration and hands-on training. Topics include: tissue-printing hybridization, dot-blot hybridization, preparation of non-radioactive probes, rapid sample extraction. After this course participants should have sufficient knowledge to introduce routine diagnostics of geminiviruses in their own laboratory.

The service offers to partners a reliable, sensitive and exhaustive technology to detect all viruses infecting a plant or fungal sample, in particular for the identification of the viruses involved in novel diseases or in syndrome of unknown etiology. The service provides counseling and costing on projects, receives the samples, performs the extraction of nucleic acids. High-throughput sequencing is outsourced to a subcontractor and the platform performs the downstream bioinformatic analysis, up to the identification of known or novel viral agents present in the samples analyzed