Virus was produced from plasmid-based DNA segments of a common Wuhan strain. The S-gene was site-specifically replaced with a cloned BA.1-based S-gene sequence.
Infectious cell culture supernatant containing SARS-CoV-2 (strain hCoV-19/Slovenia/11454/21 (lineage B 1.1.7, exUK)). This strain is preserved in viral storage medium -80°C.