Human 2019-nCoV Isolate


EVAg

Produced by
: CUB
Shipping From
: Berlin - DE

Product Description

Ref-SKU:
026V-03883
Infectious cellculture supernatant of human 2019-nCoV
Product Risk Group: 
Virus name: 
Strain: 
Isolate: 

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Complete genome
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 vial, Titer: 2,2e6 PFU / ml

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Previous Name or Taxonomy: 
Shipping conditions: 
IATA Classification: 
Infectivity Test: 
Infection with MOI of 0,001 leads to a peak in viral load within 48 to 72 hours
Viral titer: 
1x10 E+5 PFU/mL
Production cell line: 
Passage: 
1
Technical recommendation: 
Growth Pro: Adherent/ culture at 37°C and 5% CO2
Note: 

Culture Medium: DMEM (Dulbecco's Modified Eagles Medium) (PAA, Cölbe, Germany) with 4.5 g/L Glucose (PAA) supplemented with 10% Foetal Bovine Serum (PAA), 1% Penicillin/Streptomycin 100 x concentrate (Penicillin 10000 U/mL, Streptomycin 10 mg/ mL) (PAA), 1% L-Glutamine 200 mM, 1% Sodium Pyruvate 100 mM (PAA), 1% MEM nonessential amino acids (NEAA) 100 x concentrate (PAA) - Subculture routine: Detachment with an EDTA/trypsin mixture (PAA) - Split Ratio: Split 1:10 twice a week

Information about the collection of the virus

Collection date: 
Wednesday, 29 January, 2020
Suspected epidemiological origin : 
Isolation host: