Training in reverse genetics method (ISA method), recently developed to reconstitute an infectious Flavivirus or Alphavirus under BSL-3 conditions.
The training comprises DNA fragments amplification by PCR; Transfection of the purified products into permissive cells; Viral culture; Molecular detection
Freeze-dried Chikungunya virus, strain UVE/CHIKV/2009/FR/15.3 WT. This strain was obtained from modified infectious clone (LR2006_OPY1; La Reunion island 2006), transfected and cultivated on Vero cells
Freeze-dried Yellow fever virus, strain UVE/YFV/1927/GN/Asibi, complete ORF sequenced . It was produced by ISA method from "AY640589" genbank sequence.
Freeze-dried Tick Borne Encephalitis Virus, strain UVE/TBEV/2013/FR/32.11 WT-PCR, complete ORF sequence. It was produced by ISA method from infectious clone derived from Oshima 5.10 strain (genbank sequence KF623542)
