EVA

RNA extracted from infected cell culture supernatant

Template for chimeric SARS-CoV-2 rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of E, orf6, orf7a and a premature stop codon at position 213 in orf3a. The VSV-G gene is introduced at the locus of orf7a. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for chimeric SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of E, orf6, orf7a, orf3a. The VSV-G gene is introduced instead of orf3a/E. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for chimeric SARS-CoV-2 rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of E, orf6, orf7a and a premature stop codon at position 213 in orf3a. The VSV-G gene is introduced upstream of N, separated by P2A. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for chimeric SARS-CoV-2 rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), with deletion of S, E, orf6, orf7a and a premature stop codon at position 213 in orf3a. The VSV-G gene is introduced at the locus of Spike. ∆E-based viruses need to be rescued and propagated on E-transcomplementing cells. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Template for recombinant SARS-CoV-2 rescue; encodes fragment D1 of SARS-CoV-2 (nt20950-25522), Wuhan lineage. Full protocol for virus recovery using the CLEVER method in (10.7554/eLife.89035).

Protein A chromatography purified Watermelon mosaic virus (WMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Turnip yellow mosaic virus (TYMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Turnip ringspot virus (TuRSV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Turnip mosaic virus (TuMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Tomato bushy stunt virus (TBSV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Tobacco rattle virus (TRV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Southern bean mosaic virus (SBMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Sonchus yellow net betanucleorhabdovirus (SYNV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Potato yellow dwarf alphanucleorhabdovirus (PYDV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Potato black ringspot virus (PBRSV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Pepper mild mottle virus (PMMoV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Pelargonium flower break virus (PFBV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Paprika mild mottle virus (PaMMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Papaya ringspot virus (PRSV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Moroccan watermelon mosaic virus (MWMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Lettuce mosaic virus (LMV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Grapevine fanleaf virus (GFLV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Eggplant mottled dwarf alphanucleorhabdovirus (EMDV) polyclonal antibodies, raised in rabbit, suitable for serological detection

Protein A chromatography purified Clover yellow mosaic virus (ClYMV) polyclonal antibodies, raised in rabbit, suitable for serological detection