carnation Italian ringspot virus full-length cDNA clone

REFERENCE STRAIN


Product Description

Ref-SKU:
029N-04145
Carnation Italian ringspot virus full-length cDNA cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of infectious RNA
Product Risk Group: 

Information on the related virus



Unit definition: 
2 micrograms plasmid DNA, allowing for the inoculation of six host plants (Nicotiana benthamiana) after T7-driven transcription

Available within 2 weeks

300,00 €
(Cost per access for Academics)

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Cloned Product: Yes
Special feature: 
Can it be used to produce GMO: 
No
Region encompassed in this Product: 
complete genome
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Fully sequenced
Sequence checked: 
Yes
Mutations: 
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations: 
in the plasmid, the 5' nucleotide of the CIRV sequence is G instead of A. This allows efficient in vitro transcription
Genbank reference: 
Nucleic Acid preparation technique: 
Plasmid amplification in Escherichia coli DH5alpha. DNA preparation obtained by alkaline lysis, phenol extraction and PEG precipitation
Storage conditions: 
Nuclease-Free Water -20C
Shipping conditions: 
IATA Classification: 
Virus host type: 

Information about the origin of the product

Recombinant product: 
This product is a recombinant product
Biological material origin: 
Synthetic origin & Natural origin