Cymbidium ringspot virus defective interfering RNA 3 (DI-3) cDNA clone


EVAg

Originator
:
Agricultural Biotechnology Center, Godollo, Hungary

Produced by
: CNR
Shipping From
: Roma - IT

Product Description

Ref-SKU:
029N-05160
Cymbidium ringspot virus DI-3 full-length cDNA cloned in plasmid pUC18, under the control of a T7 promoter, allowing for transcription of biologically active RNA
Product Risk Group: 

Information on the related virus



Unit definition: 
1 microgram plasmid DNA, allowing for transformation and/or T7-driven transcription

In stock

200,00 €
(Cost per access for Academics)

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Cloned Product: Yes
Can it be used to produce GMO: 
No
Region encompassed in this Product: 
complete DI-3 sequence
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Fully sequenced
Sequence checked: 
Yes
Mutations: 
Contain mutations (no frameshift, no unexpected STOP codon)
Observed mutations: 
in the plasmid, the 5' nucleotide of the DI-3 sequence is G instead of A. This allows efficient in vitro transcription. The clone contains two engineered restriction sites, HpaI and EcoRV, which do not affect DI RNA replication
Nucleic Acid preparation technique: 
Plasmid amplification in Escherichia coli DH5alpha. DNA preparation obtained by Euroclone Spinnaker Plasmid DNA miniprep kit.
Storage conditions: 
Nuclease-Free Water -20C
Shipping conditions: 
IATA Classification: 
Virus host type: 

Information about the origin of the product

Recombinant product: 
This product is a recombinant product
Biological material origin: 
Synthetic origin & Natural origin