pNL-Rod-Pr


Product Description

Ref-SKU:
006N-01920
recombinant HIV-1 virus derived from pNL4-3 carrying (instead of the HIV-1 protease) the protease gene of HIV-2 (ROD14)
Product Risk Group: 

Information on the related virus

Subspecies: 
Genotype: 


Unit definition: 
10 µg purified DNA in TE Buffer

In stock

675,00 €
(Cost per access for Academics)

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Cloned Product: Yes
Can it be used to produce GMO: 
Yes
Biosafety restrictions: 
Plasmid DNA itself is not infectious unless transfected into suitable cells; current studies suggest that the construct leads to an abortive infection
Region encompassed in this Product: 
entire virus genome
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Fully sequenced
Sequence controlled: 
Yes
Mutations: 
No mutation compared to the reference sequence
Nucleic Acid preparation technique: 
solid phase ion exchange column purification
Storage conditions: 
TE Buffer -20C
Shipping conditions: 
IATA Classification: 
Note: 

The recombinant plasmid has been generated by replacing in frame the original HIV-1 protease gene of NL4-3 with the entire protease gene taken from HIV-2 ROD. The resulting plasmid expresses, upon transfection of human cells, all viral proteins but failed to produce infectious particles in experimental cell infections in vitro.