T7-driven tetracistronic Ebola virus trVLP system expressing no glycoprotein and with a Nano-luciferase reporter (pT7.1-4cis-EBOV-vRNA-dGP-nluc), recombinant non-infectious system Ebola virus Mayinga (8 plasmids)


EVAg

Produced by
: FLI
Shipping From
: Greifswald - DE

Product Description

Ref-SKU:
022N-03920
Reverse genetics-based life cycle modelling system that can serve as negative control and is best used together with other trVLP systems that allows modelling of Ebola virus genome replication and transcription, viral protein synthesis, virus assembly, budding, and entry into target cells under BSL1 conditions. Consists of 1) a plasmid encoding a T7-driven tetracistronic minigenome encoding a Nano luciferase reporter and the Ebola virus proteins VP40 and VP24; 2) expression plasmids for the Ebola virus protein NP, VP35, VP30, and L; and 3) expression plasmids for the accessory proteins T7 polymerase (for initial transcription of the minigenome), Tim1 (attachment factor) and firefly luciferase (as control). Allows insertion of other glycoprotein ORFs through two BsmBI sites. Access is restricted to non-commercial research institutions only. Further descriptions of the different trVLP systems can be found at https://www.fli.de/en/institutes/institute-of-molecular-virology-and-cell-biology-imvz/laboratories/laboratory-for-molecular-biology-of-filoviruses/
Product Risk Group: 

Information on the related virus

Virus name: 


Unit definition: 
8x 10 µl

In stock

900,00 €
(Cost per access for Academics)

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Cloned Product: Yes
Can it be used to produce GMO: 
Yes
Biosafety restrictions: 
The risk group classification of this life cycle modelling system can differ from country to country!
Region encompassed in this Product: 
Ebola virus NP, VP35, VP30, L, VP40, VP24 open reading frames, Ebola virus terminal leader and trailer regions, selected Ebola virus untranslated/non-coding regions (NP/VP35, VP40/GP, VP30/VP24), Nano and firefly luciferase open reading frames, T7 RNA polymerase open reading frame (codon optimized), and Tim1 open reading frame (codon optimized)
Plasmid: 
Plasmid selection: 
TAG: 
Sequencing: 
Partly sequenced
Sequence controlled: 
Yes
Mutations: 
No mutation compared to the reference sequence
Identification technique: 
sequencing
Storage conditions: 
Nuclease-Free Water -20C
Shipping conditions: 
IATA Classification: