EVAg

Human Immunodeficiency Virus (HIV) drug resistance remains a significant health issue that challenges treatment and prevention. The objective of this 3-week course is to learn bioinformatics techniques for the analysis of next-generation HIV sequencing results. This covers both HIV-1 and HIV-2. Teaching objectives include data curation, alignment, phylogenetics for typing, identification of drug resistance mutations, production of a resistance score, and interpretation. This course does not include genotyping itself, only sequence analysis and interpretation, as described above.

Virus Neutralization Tests (VNT), also including plaque-reduction and foci-inhibition assays like PRNT and RFFIT, are the gold standard for determining antibody titers against viruses, and are essential serological tools. In this 5-day training the application and basic principles of VNTs will be discussed, and will be combined with wet lab training. The wet lab training will include all steps of the VNT, including analysis and interpretation of the results.