DOBV-SK FD


EVAg

Produced by
: BMC-SAS
Shipping From
: Bratislava - SK

Product Description

Ref-SKU:
008V-03724
Dobrava-Belgrade virus strain SK/Aa. This Dobrava-Belgrade virus[Slovakia/Aa] is freeze-dried.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group: 
Strain: 
Genotype: 

Storage conditions: 
Freeze Dried
Sequencing: 
Partial sequence
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 ml (2 vials of 0.25 ml)

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Shipping conditions: 
IATA Classification: 
Infectivity Test: 
Focus assay
Viral titer: 
10000 FFU/ml
Production cell line: 
Technical recommendation: 
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,001 can be used for the infection and the infectious medium can be harvested 7 days after infection.

Information about the collection of the virus

Collection date: 
Friday, 12 November, 2010
Suspected epidemiological origin :