Infectious cell culture supernatant containing HIV-1 subtype F1 strain HIV-1_IoIo_F1_INMI. The strain was obtained after co-culture of patient PBMC with CD8-depleted PBMC from healthy blood donors. This strain strain is preserved under Viral Storage Medium -80C and is Mycoplasma free. To confirm its identity the virus has been partially sequenced.
Product Risk Group:
Subtype F1. Mutations: pr-K20R, pr-M36I, pr-L63T, pr-L89M.
Viral Storage Medium -80C
Infectivity tested and quantified
Virus production after co-culture of patient PBMC with CD8-depleted donor PBMC. Virus production in the supernatant was tested with p24 ELISA
Production cell line:
PCR and sequencing