Human immunodeficiency virus 2 - RH2.14-1A6


EVAg

Produced by
: ERASMUS-MC
Shipping From
: Rotterdam - NL

Product Description

Ref-SKU:
011V-02818
This clone belongs to a unique set of HIV-2 biological clones from West African immigrants in The Netherlands. Clones were obtained from patients with non-progressive, aviremic or progressive, viremic HIV-2 infection. The HIV-2 biological clones were isolated after limiting dilution co-culture of patient PBMC or plasma with CD8-depleted PBMC from healthy blood bank donors. The clone is replication competent and has been characterized for in vitro replication kinetics and co-receptor usage. Sequence is available in Genbank.
Product Risk Group: 
Subspecies: 
type 2
Strain: 
RH2.14-1A6
Isolate: 
Human immunodeficiency virus type 2 subtype A, RH2.14-1A6, Rotterdam, Erasmus MC, human, from chronic infection
Genotype: 
Human immunodeficiency virus type 2 subtype A
Serotype: 
Human immunodeficiency virus type 2 subtype A

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Complete coding sequence
Infectivity: 
Infectivity tested
Mycoplasmic content: 
Not tested

Unit definition: 
Vial with 1 ml infectious culture supernatant.

Available after 3 months

1 500,00 €
(Cost per access for Academics)

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GMO: 
No
Biosafety restrictions: 
BSL3
department of Virology, Erasmus MC, Rotterdam, The Netherlands, Originator name or acronym: Erasmus MC, Viroscience lab
Infectivity Test: 
virus production after co-culture of CD8-depleted patient PBMC with CD8-depleted donor PBMC. Virus production in the supernatant was tested with HIV-2 viral RNA qPCR, plus TZA assay
Production cell line: 
Genbank reference: 
Collection date: 
Friday, 1 June, 2001
Isolation host: 
Isolation technique: 
infection of donor PBMC
Isolation conditions: 
infection of CD8 depleted donor PBMC
Clinical information: 
non-progressive HIV-2 infection, plasma viral load <500 copies/ml, CD4 T cell count 550 cells/uL, cART naïve,
Passage: 
Low passage number (estimated 3 cell free passages)
Identification technique: 
HIV-2 qPCR on culture supernatant
Shipping conditions: 
IATA Classification: