LGTV


EVAg

Produced by
: BMC-SAS
Shipping From
: Bratislava - SK

Product Description

Ref-SKU:
008v-EVA356
Langat virus - original isolate. This Langat virus is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group: 
Virus name: 

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Complete genome
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 ml (2vials of 0,5 ml)

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Infectivity Test: 
Infectivity was proven by plaque titration. Infectious titre was determined to be 1x 10^6 PFU/ml.
Viral titer: 
1000000 PFU/ml
Production cell line: 
Shipping conditions: 
Passage: 
Passaged 18 times on suckling mice in 1988 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 6th June 2011.
IATA Classification: 
Identification technique: 
RT-PCR followed by sequencing of the amplified product.
Technical recommendation: 
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle`s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,001 can be used for the infection and the infectious medium can be harvested 10 days after infection.

Information about the collection of the virus

Collection date: 
Wednesday, 23 May, 2018