PUUV Sotkamo


EVAg

Produced by
: BMC-SAS
Shipping From
: Bratislava - SK

Product Description

Ref-SKU:
008v-EVA1472
Puumala virus, strain Sotkamo. This Puumala virus[Sotkamo] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group: 
Virus name: 
Strain: 
Sotkamo

Storage conditions: 
Viral Storage Medium -80C
Sequencing: 
Partial sequence
Infectivity: 
Infectivity tested and quantified
Mycoplasmic content: 
Mycoplasma free

Unit definition: 
1 ml

In stock

2 000,00 €
(Cost per access for Academics)

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GMO: 
No
Infectivity Test: 
Infectivity was proven by focus assay. Infectious titre was determined to be 3,3 x 10^5 FFU/ml.
Viral titer: 
330000 FFU/ml
Production cell line: 
Collection date: 
Friday, 12 November, 2010
Passage: 
Recent stock prepared by one passage on Vero E6 cells on 19th November 2010.
Identification technique: 
RT-PCR followed by sequencing of the amplified product.
Technical recommendation: 
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle"s BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165).
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IATA Classification: