Semliki forest virus original isolate. This Semliki forest virus[original.] is preserved under Viral Storage Medium -80C.Tests for the presence of mycoplasmae were negative. To confirm its identity the virus has been partly sequenced.
Product Risk Group:
Viral Storage Medium -80C
Infectivity tested and quantified
Infectivity was proven by plaque titration. Infectious titre was determined to be 8x 10^7 PFU/ml.
Production cell line:
Passaged 18 times on suckling mice in 1986 and stored freeze-dried. New stock prepared by one passage on Vero E6 cells on 8th december 2010.
RT-PCR followed by sequencing of the amplified product
We recommend to grow the virus on VeroE6 cells cultivated in MEM Eagle with Earle's BSS (BioWhittaker, 12-136F). Following additives should be added to 500 ml of medium: 5ml Nonesential amino acids (Biochrom AG, K0293), 5 ml Sodium Pyruvate 100mM, (Biochrom AG, L0473), 5 ml L-Alanyl-L-glutamine 200mM (Biochrom AG, K0302), 5 ml Penicilin/ Streptomycin/ Amphotericin (BioWhittaker 17-745E), 25 ml FBS heat inact.s.American (Invitrogen, 10108-165). Multiplicity of infection (MOI) of 0,001 can be used for the infection and the infectious medium can be harvested 50 hours after infection.
Information about the collection of the virus
Biological material origin:
Collection date: BEFORE
Wednesday, 29 December, 1993
Country of collection: